Anti-Human IL-10 Rβ (Clone 90220)

Product No.: I-715


Clone 90220 Target IL-10 Rβ Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-10 Receptor Beta, IL10RB, CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2 Isotype IgG1 Applications CyTOF® , FC , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen NS0-Derived Recombinant Human Interleukin-10 Receptor β Extracellular Domain (Accession # Q08334) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830836 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-10 Rβ. A suggested method would be to stain cells expressing IL-10 Rβ with 0.25 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of ≤200 µl followed by PN:M1189. Western Blotting: To detect Human IL-10 Rβ this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for rhIL-10 Rβ is approximately 50 ng/lane and 100 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human IL-10 Rβ bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-10 Rβ (at 0.25 ng/ml) is 0.5 - 2 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Interleukin 10 Receptor Beta (IL-10 Rβ) (Clone 90220) recognizes an epitope on Human IL-10 Rβ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Interleukin 10 receptor, beta subunit (IL10RB) is a subunit for the interleukin-10 receptor which has recently been designated CDW210B (cluster of differentiation W210B). The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. PubMed IL-10 Rβ NCBI Gene Bank ID 3588 UniProt.org Information on Uniprot.org References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols CyTOF® N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

NS0-Derived Recombinant Human Interleukin-10 Receptor β Extracellular Domain (Accession # Q08334)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830836

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-10 Rβ. A suggested method would be to stain cells expressing IL-10 Rβ with 0.25 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of ≤200 µl followed by PN:M1189.
Western Blotting: To detect Human IL-10 Rβ this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for rhIL-10 Rβ is approximately 50 ng/lane and 100 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human IL-10 Rβ bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-10 Rβ (at 0.25 ng/ml) is 0.5 - 2 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Interleukin 10 Receptor Beta (IL-10 Rβ) (Clone 90220) recognizes an epitope on Human IL-10 Rβ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Interleukin 10 receptor, beta subunit (IL10RB) is a subunit for the interleukin-10 receptor which has recently been designated CDW210B (cluster of differentiation W210B). The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction.

PubMed

IL-10 Rβ

NCBI Gene Bank ID

3588

UniProt.org

Information on Uniprot.org