Anti-Human IL-17R
Anti-Human IL-17R
Product No.: I-627
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Clone 133617 Target IL-17R Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-17 Receptor, IL17R, CDw217, IL-17RA, IL17RA, MGC10262 Isotype IgG1 Applications CyTOF® , FC , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human IL-17R (Accession # Q96F46) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830749 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-17R. A suggested method would be to stain cells expressing IL-17R with 0.25 µl per 1-2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:M1189. Western Blotting: To detect Human IL-17R this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-17R is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-552. Additional Applications Reported In Literature ? Neutralization: At 2 μg/mL, this antibody will neutralize 70 - 90% of human IL-17 receptor-mediatedbioactivity in the presence of 25 ng/mL of rhIL-17 using IL-17-dependent secretion of IL-6by the NHDF cell line as an assay. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Interleukin 17 Receptor (IL-17R) (Clone 133617) recognizes an epitope on Human IL-17R. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Interleukin-17 receptor (IL-17R) Type 1 transmembrane glycoprotein which binds interleukin 17. IL-17R signaling is critical for pulmonary neutrophil recruitment and host defense against Gram-negative bacteria.1 It represents a marker for the metastasizing ability of osteosarcoma.1 Antigen DetailsPubMed References & Citations1. Facchini, A. et al. (2007) J clin. Research clin. oncol. 133: 1017 2. Armitage, RJ. et al.(1997) Cytokine. 9(11):794-800. Technical ProtocolsCyTOF®  N  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
