Anti-Human IL-1RAP (IL-1R3) (Nadunolimab)

Anti-Human IL-1RAP (IL-1R3) (Nadunolimab)

Product No.: I-2140

- -
- -
Product No.I-2140
Clone
CAN04
Target
IL-1RAP
Product Type
Biosimilar Recombinant Human Monoclonal Antibody
Alternate Names
IL-1R-3, IL-1R3, IL-1RAcP
Isotype
Human IgG1κ
Applications
Act
,
Antagonist
,
B
,
ELISA
,
FA
,
IHC

- -
- -
Select Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Human
Host Species
Human
Expression Host
HEK-293 Cells
FC Effector Activity
Active
Immunogen
Fusion protein of the extra cellular part of IL-1RAP (Pro100-Lys330)
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% by SDS Page
≥95% monomer by analytical SEC
Formulation
This biosimilar antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
Recombinant biosimilar antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade biosimilar antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
ACT,
Antagonist,
B,
ELISA,
FA,
IHC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Nadunolimab. Nadunolimab (CAN04) activity is directed against IL- 1RAP.
Background
IL-1RAP (interleukin-1 receptor accessory protein) is a co-receptor that works with the IL-36 subfamily of the IL-1 interleukin family1. Members of the IL-1 family play key signaling roles in innate and adaptive immunity. IL-1RAP is particularly involved in promoting inflammatory responses via the IL-1, IL-33, and IL-36 signaling pathways. IL-1RAP also plays a role in cancer promotion, progression, and metastasis. Additionally, IL-1RAP dimerizes with IL-1R1 to initiate signaling of IL-1α and IL-1β, both of which promote chemotherapy resistance2. As such, IL-1RAP is a target for cancer therapy1. Two different therapies targeting IL-1RAP are under development: 1) chimeric antigen receptor T-cells (CAR-T) therapy and 2) antibody immunotherapy for either direct blockade or activation of antibody-dependent cell-mediated cytotoxicity (ADCC).

Nadunolimab was generated by grafting the complementarity-determining regions of a murine monoclonal antibody into a human IgG1 isotype3. BALB/c mice were immunized with a fusion protein of the extracellular part of IL-1RAP (Pro100-Lys330) and the Fc-part of human IgG1. Splenocytes were fused with mouse myeloma cell line Sp2/0 and screened by ELISA for binding to the IL-1RAP extracellular domain3,4.

Nadunolimab binds IL-1RAP in a manner that blocks IL-1α and IL-1β as well as partially inhibits IL-33 signaling. Nadunolimab binds to the top of the IL-1RAP D2 domain, specifically to residues 105-114, 145-158, and 169-1765. Residues Thr154 to Ile171 are necessary for binding, with Q165 being critical. Additionally, binding is directly coincident with portions of the IL-1β and IL-33 signaling complexes.

Alternative names for nadunolimab are CAN04 and mAb3F8.

Antigen Distribution
IL-1RAP is ubiquitously expressed. On normal blood cells, IL-1RAP is expressed mainly on monocytes. IL-1RAP is overexpressed on tumor cells of several hematological and solid cancers. IL-1RAP has both membrane-bound and soluble forms.
Ligand/Receptor
IL-36, IL-1, IL-1R1
NCBI Gene Bank ID
UniProt.org
Research Area
Cancer
.
Immuno-Oncology
.
Inflammatory Disease

References & Citations

1 Frenay J, Bellaye PS, Oudot A, et al. Int J Mol Sci. 23(23):14918. 2022.
2 Rydberg Millrud C, Deronic A, Grönberg C, et al. Cancer Immunol Immunother. 72(3):667-678. 2023.
3 Ågerstam H, Karlsson C, Hansen N, et al. Proc Natl Acad Sci U S A. 112(34):10786-10791.2015.
4 Askmyr M, Ågerstam H, Hansen N, et al. Blood. 121(18):3709-3713. 2013.
5 Fields JK, Kihn K, Birkedal GS, et al. Front Immunol. 12:779100. 2021.
6 Robbrecht D, Jungels C, Sorensen MM, et al. Br J Cancer. 126(7):1010-1017. 2022.
7 Ågerstam H, Hansen N, von Palffy S, et al. Blood. 128(23):2683-2693. 2016.
Act
Antagonist
B
Indirect Elisa Protocol
FA
IHC

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.