This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human IL-1 Receptor-Associated Kinase (IRAK) recognizes Human, Mouse and Rat IRAK. This polyclonal antibody was purified using affinity chromatography.
Nuclear factor κ B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).
References & Citations
1. Cao, Z. et al. (1996) Science 271:1128
2. Trofimova, M. et al. (1996) J. Bio. Chem. 271:17609
3. Jianing, H. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12829
4. Robinson, D. et al. (1997) Immunity 7:571
Products are for research use only. Not for use in diagnostic or therapeutic procedures.