Anti-Human Lamin A/C (Clone 4C11) – Biotin
Anti-Human Lamin A/C (Clone 4C11) – Biotin
Product No.: L352
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Clone 4C11 Target Lamin A/C Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names Prelamin-A/C, 70 kDa lamin, renal carcinoma antigen NY-REN-32, LMNA Isotype Mouse IgG2a k Applications IF Microscopy , IP , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Wild-type Ig-fold domain of lamin A/C (aa 430–545) Product Concentration 0.5 mg/ml Formulation This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. State of Matter Liquid Storage and Handling This biotinylated antibody is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? Dot, IF Microscopy, IP, WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity 4C11 activity is directed against human lamin A/C. Background The nucleoplasm is separated from the cytoplasm by a bilayer nuclear envelope that consists of
outer and inner nuclear membranes, nuclear pore complexes, and the nuclear lamina, which is a
two-dimensional matrix composed mainly of type V intermediate filament proteins called lamins
1. Lamins are encoded by a family of three genes that collectively produce lamins B1, B2, B3,
AΔ10, C2, and A/C. Lamin A/C plays a variety of roles in the nucleus including, but not limited
to: nuclear mechanical stability, structural maintenance, positioning, chromatin organization,
epigenetic regulation, transcription, DNA replication, cell cycle progression, differentiation,
migration, and polarization. Additionally, lamin A/C is variably expressed in human immune
cells during different stages of cell development and activation states. 4C11 was raised against the wild-type Ig-fold domain of lamin A/C (aa 430–545)2. This domain is located in the C-terminal globular domain of the protein’s tripartite structure. A BALB/c mouse was immunized with antigen and splenocytes fused with myeloma cell line X63Ag8.653. 4C11 detects both lamins A and C. Antigen Distribution Lamin A/C is abundantly expressed in most differentiated cells but not
pluripotent stem cells and embryos during development. Lamin A/C is expressed at high levels
in macrophages and dendritic cells but is barely detectable in inactivated T and B cells.
Neutrophils lose lamin A/C expression during maturation. T cells exhibit a transient peak in
lamin A/C upon antigen recognition. Some B cell lymphomas express lamin A/C. Ligand/Receptor N/A NCBI Gene Bank ID UniProt.org Research Area Adaptive Immunity . Cell Biology . Cell Motility/Cytoskeleton/Structure . Innate Immunity References & Citations1. Saez A, Herrero-Fernandez B, Gomez-Bris R, et al. Int J Mol Sci. 21(17):6109. 2020. 2. Roblek M, Schüchner S, Huber V, et al. PLoS One. 5(5):e10604. 2010. 3. Schüchner S, Andorfer P, Mudrak I, et al. Sci Rep. 6:31363. 2016. Technical ProtocolsIF Microscopy   Certificate of Analysis |
Formats Available
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Prod No. | Description |
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L351 | |
L352 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
