Anti-Human Lamin A/C (Clone 4C11) – Purified No Carrier Protein

Wild-type Ig-fold domain of lamin A/C (aa 430–545)

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

Purified antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

4C11 activity is directed against human lamin A/C.

The nucleoplasm is separated from the cytoplasm by a bilayer nuclear envelope that consists of outer and inner nuclear membranes, nuclear pore complexes, and the nuclear lamina, which is a two-dimensional matrix composed mainly of type V intermediate filament proteins called lamins ¹. Lamins are encoded by a family of three genes that collectively produce lamins B1, B2, B3, AΔ10, C2, and A/C. Lamin A/C plays a variety of roles in the nucleus including, but not limited to: nuclear mechanical stability, structural maintenance, positioning, chromatin organization, epigenetic regulation, transcription, DNA replication, cell cycle progression, differentiation, migration, and polarization. Additionally, lamin A/C is variably expressed in human immune cells during different stages of cell development and activation states.

4C11 was raised against the wild-type Ig-fold domain of lamin A/C (aa 430–545)². This domain is located in the C-terminal globular domain of the protein’s tripartite structure. A BALB/c mouse was immunized with antigen and splenocytes fused with myeloma cell line X63Ag8.653. 4C11 detects both lamins A and C.

Lamin A/C is abundantly expressed in most differentiated cells but not pluripotent stem cells and embryos during development. Lamin A/C is expressed at high levels in macrophages and dendritic cells but is barely detectable in inactivated T and B cells. Neutrophils lose lamin A/C expression during maturation. T cells exhibit a transient peak in lamin A/C upon antigen recognition. Some B cell lymphomas express lamin A/C.

N/A

1. Saez A, Herrero-Fernandez B, Gomez-Bris R, et al. Int J Mol Sci. 21(17):6109. 2020.
2. Roblek M, Schüchner S, Huber V, et al. PLoS One. 5(5):e10604. 2010.
3. Schüchner S, Andorfer P, Mudrak I, et al. Sci Rep. 6:31363. 2016.