Anti-Human LIF

Pricing & Details

Product No.L156
Protein
LIF
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Leukemia Inhibitory Factor, CDF, DIA
Applications
IHC FFPE
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
L156-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
L156-100 µg
100 µg
$445.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Goat
Immunogen
E. coli-derived recombinant human LIF (Acession No.: P15018)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml.
Neutralization: Measured by its ability to neutralize LIF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.04-0.08 µg/mL in the presence of 3 ng/mL Recombinant Human LIF.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Leukemia Inhibitory Factor recognizes Human LIF by ELISA. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. By ELISAs, less than 50% cross-reactivity with recombinant mouse LIF was observed.
Antigen Distribution
Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells.
Background
Leukemia inhibitory factor (LIF) (22 kDa (predicted) glycoprotein) is a pleiotropic cytokine that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190, and the subunit gp130. Human LIF acts through a receptor comprising a 190 kDa LIF-binding alpha -chain and a 130 kDa signal-transducing beta -chain (gp130), which is shared with the other members of the IL-6 family.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
IHC
Prod No.
Description
L156
A323
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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