Anti-Human MCP-2 (Clone 35509)
Pricing & Details
Formats AvailableView All
SCYA8, CCL8, HC14, SCYA10, Small Inducible Cytokine A8, Chemokine (C-C Motif) Ligand 8, Monocyte Chemotactic, C-C Motif Chemokine 8
Purified Recombinant Human MCP-2 (>98%)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:M124 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:M157.
Western Blotting: To detect Human MCP-2 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MCP-2 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M157.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human MCP-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MCP-2 (at 0.25 ng/ml) is 1 - 4 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Monocyte Chemotactic Protein-2 (MCP-2) (Clone 35509) recognizes an epitope on Human MCP-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
MCP-2 is a non-glycosylated polypeptide that is a member of the C-C chemokine subfamily and it specifically attracts monocytes, lymphocytes, basophils and eosinophils. It also plays a role in neoplasia and inflammatory host responses. MCP-2 can bind heparin.1
References & Citations
1. Van Damme J. et al. (1998) J. Immunol. 160: 4034
Products are for research use only. Not for use in diagnostic or therapeutic procedures.