Anti-Human HLA-DP (MHC Class II) Monomorphic [Clone B7/21] — Purified in vivo GOLD™ Functional Grade

Anti-Human HLA-DP (MHC Class II) Monomorphic [Clone B7/21] — Purified in vivo GOLD™ Functional Grade

Product No.: H260

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Clone
B7/21
Target
HLA-DP Monomorphic
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
HLA-DP Monomorphic, HLADP, HLA-DPαβ, DPA and DPB, DPα and DPβ, DPαβ
Isotype
Mouse IgG3
Applications
FC
,
ICC

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Recommended Isotype Controls
Mouse IgG3
Recommended Dilution Buffer
Immunogen
Unknown
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Additional Applications Reported In Literature ?
ICC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone B7/21 recognizes a monomorphic epitope on human HLA-DP1, -DP2, -DP3, -DP4, and -DP5. It does not cross-react with HLA-DR or HLA-DQ.
Background
HLA-DP antibody, clone B7/21, recognizes the major histocompatibility complex (MHC) class II molecule Human Leukocyte Antigen - DP isotype (HLA-DP). MHC class II is constitutively expressed on human professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation1. HLA-DP consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain2 encoded by the HLA-DPA1 and HLA-DPB1 genes, respectively, located in the HLA complex of chromosome 6. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens3. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling4. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation5 and can transduce signals into T cells, enhancing T cell proliferation and activity6. High HLA-DP expression is associated with an increased risk of graft-versus-host disease7. Specific alleles of HLA-DP are associated with autoimmune diseases, including rheumatoid arthritis8.
Antigen Distribution
HLA-DP is expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells.
Research Area
Immunology
.
Innate Immunity

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Clone B7/21 is a monoclonal antibody that specifically recognizes a monomorphic epitope on human HLA-DP molecules, predominantly used in flow cytometry and related applications to analyze human cells. There is no evidence in the provided search results that B7/21 is used directly for in vivo mouse studies.

Key context and application details:

  • Species specificity: B7/21 targets multiple human HLA-DP isotypes (DP1, DP2, DP3, DP4, DP5) and does not cross-react with the mouse MHC class II equivalents, such as I-A or I-E. Therefore, it is not suitable for targeting murine MHC molecules in mouse models.
  • Typical use: The antibody is routinely used to stain human leukocytes (especially antigen-presenting cells) in flow cytometric analysis, helping to assess human HLA-DP expression after transplantation or in humanized mouse models containing human immune cells.
  • Recombinant forms: Modern versions use human IgG1 constant regions to enhance downstream applications, but their specificity for human HLA-DP remains unchanged.
  • In vivo mouse studies: The only possible in vivo mouse application would be in “humanized mouse” models, where mice are engrafted with human immune cells or tissues expressing HLA-DP. In these cases, B7/21 could be employed post-mortem to identify and characterize human cells within mice, but not to manipulate or track murine cells themselves.

Summary Table: Specificity and In Vivo Use of B7/21

CategoryDescription
Target epitopeHuman HLA-DP (not mouse MHC)
Cross-reactivityNo cross-reactivity with mouse MHC II (I-A & I-E)
Typical applicationsFlow cytometry of human cells, analysis of human APCs
In vivo mouse useOnly useful in humanized mouse models, not standard mouse immunology studies

If your aim is to study MHC class II molecules in murine models, you should use antibodies specific to mouse MHC II (e.g., anti-I-A, anti-I-E clones). Clone B7/21 is not appropriate for direct immunological studies in mice unless the mice contain human cells expressing HLA-DP.

Commonly used antibodies or proteins paired with B7/21 (which targets HLA-DP, a human MHC Class II antigen) in the literature often include other MHC Class II antibodies and markers for immune cell characterization. The most frequent combinations are:

  • HLA-DR and HLA-DQ antibodies: Since B7/21 is highly specific for HLA-DP and does not react with HLA-DR or HLA-DQ, studies often use anti-HLA-DR and anti-HLA-DQ antibodies alongside B7/21 to distinguish between different MHC class II molecules on the cell surface or tissue sections.

  • CD markers (e.g., CD4, CD8, CD14, CD19): These antibodies are frequently used to identify immune cell types expressing or presenting HLA-DP. For example, CD4 identifies helper T cells, CD19 marks B cells, and CD14 marks monocytes/macrophages—all of which can express MHC class II antigens, including HLA-DP.

  • Other B7 family proteins: Research regularly employs antibodies against PD-L1 (B7-H1), B7-H3, and related costimulatory molecules to examine their roles in immune modulation and tumor immunology, particularly in the context of cancer studies or immune checkpoint exploration. For instance, B7-H3 is frequently studied in combination with CD3 (T cell marker) and Fc-modified antibodies for functional assays or therapeutic applications.

  • Treg and T cell activation markers: In studies involving immune regulation or tumor immunity, antibodies against CD25 (a Treg marker), CTLA-4, and related proteins are used alongside B7 molecules to evaluate immune cell activation or suppression. For example, combining B7-Fc fusion proteins (such as B7.1-Fc) with anti-CD25 (PC61) is a common approach in tumor immunotherapy animal models.

Frequently Combined Antibodies/ProteinsContext of Use
Anti-HLA-DR, Anti-HLA-DQDistinguishing MHC class II subtypes
CD4, CD8, CD14, CD19, CD3Immunophenotyping of T cells, B cells, macrophages
PD-L1 (B7-H1), B7-H3 antibodiesCo-stimulatory/immune checkpoint molecule analysis
CD25 (PC61), CTLA-4Treg depletion, T cell regulation, combined immunotherapy
B7.1-Fc fusion proteinFunctional/therapeutic studies with anti-tumor activity

The exact combination depends on the experimental or clinical context—such as flow cytometry panels, immunohistochemistry, or functional immune assays. The use of switch-variant B7/21 (IgG1 vs. IgG3) is another detail sometimes explored for blocking or cross-blocking experiments in studies of HLA-DP specificity.

In summary, anti-HLA-DR, anti-HLA-DQ, immue cell-type markers (CD4, CD8, CD14, CD19), and various co-signaling molecule antibodies (PD-L1, B7-H3, CD25, CTLA-4) are most commonly used in conjunction with B7/21 in the literature.

Clone B7/21 is a well-established monoclonal antibody that specifically recognizes a monomorphic epitope on human HLA-DP and is widely used in immunological studies to identify or block HLA-DP molecules. Key findings from scientific literature citing clone B7/21 include:

  • Specificity: Clone B7/21 binds specifically to human HLA-DP1, -DP2, -DP3, -DP4, and -DP5, but it does not cross-react with other MHC class II molecules such as HLA-DR or HLA-DQ. This makes it valuable for distinguishing HLA-DP from other MHC class II proteins in experimental settings.

  • Applications:

    • It is widely used in flow cytometry, immunoprecipitation, and blocking studies to study the distribution, structure, and function of HLA-DP on various cell types, especially professional antigen-presenting cells (APCs) like B cells, dendritic cells, and macrophages.
    • The antibody can aid in examining conditions and mechanisms involving HLA-DP presentation, such as T-cell responses, transplantation compatibility, and some autoimmune disease contexts.
  • Role in Research on Class II MHC Functions: Clone B7/21 is often utilized in mechanistic studies to define the contribution of HLA-DP to antigen presentation and to differentiate responses mediated via DR, DQ, or DP molecules. In one cited immunological study, B7/21 was used to show that certain T cell clones were not activated via classical DR, DQ, or DP molecules, suggesting the presence of novel class II-like molecules; here, B7/21 was used specifically to rule out HLA-DP as the relevant stimulating determinant.

  • Engineering and Improvements: Recombinant versions of B7/21 have been developed, often switching the constant region from mouse IgG3 (which tends to precipitate) to human IgG1 for improved performance and compatibility with human systems in research and potential clinical use.

There is no evidence in these results that clone B7/21 is associated with functional signaling upon binding (i.e., it does not appear to modulate HLA-DP function, but serves rather as a blocking or detection reagent).

Accordingly, the key scientific contributions of clone B7/21 are its robust specificity for HLA-DP and its broad experimental utility in immunology for distinguishing or functionally interrogating HLA-DP in both basic and translational research.

Dosing regimens for clone B7/21, a monoclonal antibody targeting human HLA-DP antigens, are not specifically outlined for in vivo mouse studies in publicly available dosing guides or experimental reports. The details available primarily describe its specificity and applications (e.g., immunocytochemistry on human cells), but do not provide mouse dosing schedules, routes, or variations across different mouse models.

Essential context:

  • The B7/21 monoclonal antibody is designed to recognize human HLA-DP molecules and is most commonly used for in vitro assays, such as immunocytochemistry (ICC), flow cytometry, or cell sorting on human samples or humanized cell lines. It does not cross-react with mouse antigens because the recognized determinants are specific to the human HLA-DP isoforms.
  • There is no evidence from current antibody dosing guides that B7/21 is used for in vivo mouse models, nor do established mouse model dosing guides list regimens for this clone. Most dosing regimens for monoclonal antibodies in mice (e.g., anti-PD-1, anti-CD4) typically fall in the 100–250 µg per mouse, administered intraperitoneally 2-3 times per week, but these are for antibodies against murine antigens.

Additional relevant information:

  • Dosing regimens may vary widely depending on pharmacokinetics, disease model, antigen expression, and study purpose (e.g., depletion, blocking, or detection).
  • For monoclonal antibodies used in preclinical mouse studies, initial starting doses generally range from 1-15 mg/kg. However, this is not directly translatable to B7/21, as it is intended for recognizing human, not murine, targets.
  • If B7/21 were used in a humanized mouse model (expressing human HLA-DP), it would be critical to optimize dose, route, and schedule empirically due to variability in antigen expression and host immune responses. No published data currently detail such regimens for B7/21.

In summary, there are no standardized or published dosing regimens for B7/21 across mouse models. Its use is limited to assays with human cells, and any adaptation for in vivo mouse studies (such as humanized mice) would require case-specific pilot dosing.

References & Citations

1. Holling TM, et al. (2004) Hum Immunol. 65(4):282-90.
2. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25.
3. Wieczorek M, et al. (2017) Front Immunol. 8:292.
4. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921.
5. Barnaba V, et al. (1994) Eur J Immunol. 24(1):71-5.
6. Di Rosa F, et al. (1993) Hum Immunol. 38(4):251-60.
7. Petersdorf EW, et al. (2015) N Engl J Med. 373(7):599-609.
8. Raychaudhuri S, et al. (2012) Nat Genet. 44(3):291-6.
Flow Cytometry
ICC

Certificate of Analysis

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