Anti-Human/Mouse Bax

Anti-Human/Mouse Bax

Product No.: B595

[product_table name="All Top" skus="B595"]

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Clone
127602
Target
Bax
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
NT
Isotype
IgG2a
Applications
ICC
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Sf 21-Derived Human Bax Minus The Carboxyl Terminal
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 1.0-2.0 µg/mL.
Additional Applications Reported In Literature ?
Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Bax (Clone 127602) recognizes an epitope on Human Bax. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer.1 The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death.2,3 Bax, a pro-apoptotic Bcl-2 family member, is a cytosolic protein that changes conformation and translocates to the mitochondria following apoptotic stimuli.4,5 It is thought to share significant functional homology with Bak, another pro-apoptotic Bcl-2 family member, as disruption of bax or bak has little effect on cell death, but mice lacking both genes display multiple developmental defects and cells lacking both show decreased apoptotic capability.

Antigen Details

PubMed
NCBI Gene Bank ID
ICC
General Western Blot Protocol
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Formats Available

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Prod No.
Description
B595
Products are for research use only. Not for use in diagnostic or therapeutic procedures.