Anti-Human/Mouse HDAC2 (Clone 3f3) – Biotin
Anti-Human/Mouse HDAC2 (Clone 3f3) – Biotin
Product No.: H456
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Clone 3F3 Target HDAC2 Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names Protein deacylase HDAC2, YY1 transcription factor-binding protein Isotype Mouse IgG1 κ Applications ICFC , IHC FFPE , WB , Chromatin IP (ChIP) |
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Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse Host Species Mouse Immunogen KLH-conjugated, synthetic peptide corresponding to the carboxy terminus of human HDAC2, amino acids 473-488 Product Concentration 0.5 mg/ml Formulation This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. State of Matter Liquid Storage and Handling This biotinylated antibody is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? Chromatin IP, ICFC, IHC FFPE, WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity 3F3 activity is directed against human and mouse HDAC2. Background Histone modifications are essential to cellular differentiation and proliferation throughout growth
and development1. Histone deacetylases induce changes in chromatin structure that enable
epigenetic control and modulation of gene expression patterns. Histone deacetylase 2 (HDAC2)
is a class I histone deacetylase that, along with HDAC1, regulates gene expression as part of a
corepressor complex. HDAC2 catalyzes the deacetylation of lysine residues on the N-terminus of
core histones H2A, H2B, H3 and H42. HDAC2 can also perform noncatalytic activities1.
Additionally, HDAC2 associates with F-actin in mitotic cells3. HDAC inhibitors are being
investigated for the treatment of neurological diseases and cancer1. 3F3 was generated by immunizing mice with a KLH-conjugated, synthetic peptide corresponding to the carboxy terminus of human HDAC2, amino acids 473-488. Antigen Distribution HDAC2 is widely expressed, with lower levels present in the brain and
lung. Ligand/Receptor HDAC1, MAD, SIN3, YY1, N-COR, RBBP4, RBBP7, among many others Research Area Cell Biology . Neuroscience . Transcription Factors . Chromatin . DNA . Epigenetics References & Citations1 Hagelkruys A, Mattes K, Moos V, et al. Mol Cell Biol. 36(3):462-474. 2015. 2 https://www.uniprot.org/uniprotkb/Q92769/entry 3 He S, Khan DH, Winter S, et al. J Cell Physiol. 228(7):1525-1535. 2013. 4 Li P, Wang D, Yao H, et al. Oncogene. 29(21):3153-3162. 2010. 5 Hagelkruys A, Lagger S, Krahmer J, et al. Development. 141(3):604-616. 2014. 6 Huang Y, Tang Q, Nguyen M, et al. Virol J. 8:151. 2011. 7 Preglej T, Hamminger P, Luu M, et al. JCI Insight. 5(4):e133393. 2020. 8 Kiweler N, Schwarz H, Nguyen A, et al. Cell Biol Toxicol. 39(5):2401-2419. 2023. Technical ProtocolsICFC IHC FFPE  Chromatin IP (ChIP) Certificate of Analysis |
Formats Available
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Prod No. | Description |
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H455 | |
H456 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
