Anti-Human/Mouse PARP

Product No.: P281


Clone 53015 Target PARP Formats AvailableView All Product Type Monoclonal Antibody Isotype IgG2a Applications WB


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Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen Amino Acid 71 - 329 of Mouse PARP Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831577 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse PARP this monoclonal antibody can be used at a concentration of 0.6 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Rat Anti-Mouse Poly ADP-Ribose Polymerase (PARP) (Clone 53015) recognizes an epitope on Mouse PARP. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The antibody also detects the 84 kDa product of PARP cleavage in human cells and 60 - 70 kDa bands generated in 3T3 cells treated with stauosporine. A cross-reactive band of 160 kDa is detected in human Jurkat cells PubMed PARP NCBI Gene Bank ID 142 UniProt.org Information on Uniprot.org References & Citations View product citations for antibody P281 on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

Amino Acid 71 - 329 of Mouse PARP

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831577

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse PARP this monoclonal antibody can be used at a concentration of 0.6 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse Poly ADP-Ribose Polymerase (PARP) (Clone 53015) recognizes an epitope on Mouse PARP. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The antibody also detects the 84 kDa product of PARP cleavage in human cells and 60 - 70 kDa bands generated in 3T3 cells treated with stauosporine. A cross-reactive band of 160 kDa is detected in human Jurkat cells

PubMed

PARP

NCBI Gene Bank ID

142

UniProt.org

Information on Uniprot.org