Anti-Human/Mouse PARP
Anti-Human/Mouse PARP
Product No.: P281
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Clone 53015 Target PARP Formats AvailableView All Product Type Monoclonal Antibody Isotype IgG2a Applications WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Amino Acid 71 - 329 of Mouse PARP Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831577 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse PARP this monoclonal antibody can be used at a concentration of 0.6 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse Poly ADP-Ribose Polymerase (PARP) (Clone 53015) recognizes an epitope on Mouse PARP. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The antibody also detects the 84 kDa product of PARP cleavage in human cells and 60 - 70 kDa bands generated in 3T3 cells treated with stauosporine. A cross-reactive band of 160 kDa is detected in human Jurkat cells Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org Technical Protocols |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
