Anti-Human NFKB2 (Clone CPTC-NFKB2-1) – Purified No Carrier Protein

NFKB2 synthetic peptide IEVDLVTHSDPPR

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

SAIC-26C-15H2/L15 activity is directed against human NFKB2 peptide sequence IEVDLVTHSDPPR.

CPTC-NFKB2-1

NFKB2 is a member of the Rel/NF-κB transcription factor family¹, which consists of five monomers that act as homo- and heterodimers involved in the transcriptional activation and repression of hundreds of genes and plays important roles in inflammation, cell physiology², and the immune system¹. NFKB2 encodes p52, which is synthesized as a large precursor protein (p100) that is processed by proteosome-mediated proteolysis to the mature, DNA-binding p52 peptide³. Truncations and chromosomal fusions in the NFKB2 gene are present in a number of B and T cell lymphomas as well as myelomas^2,3. Aberrant peptides resulting from these mutations abolish the inhibitory function of p52, leading to constitutive DNA binding activity and malignancy². The precursor p100 protein also fulfills important biological functions by acting as an inhibitor of most other members of the NF-κB family.

SAIC-26C-15H2/L15 was generated in rabbit for use in immuno-MRM assays using a synthetic peptide derived from human NFKB2, IEVDLVTHSDPPR⁴. SAIC-26C-15H2/L15 can also detect NFKB2 in indirect ELISA assays⁵. SAIC-26C-15H2/L15 does not detect NFKB2 by immunohistochemistry, immunofluorescence, or Western blotting.

NFKB2 is a subunit of the NFkB transcription factor complex present in almost all cell types.

NFKBIE, BCL3, MEN1

1 Vallabhapurapu S, Karin M. Annu Rev Immunol. 27:693-733. 2009.
2 Ghosh G, Wang VY. Front Cell Dev Biol. 9:764164. 2021.
3 Courtois G, Gilmore TD. Oncogene. 25(51):6831-6843. 2006.
4 Whiteaker JR, Lundeen RA, Zhao L, et al. Front Immunol. 12:765898. 2021.
5 https://antibodies.cancer.gov/detail/CPTC-NFKB2-1