Anti-Human p34cdc2 Serine/Threonine Kinase – Alexa Fluor® 647

Purified Recombinant Human p34^cdc2 Serine/Threonine Kinase (>98%)

This Alexa Fluor® 647 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Alexa Fluor® is a trademark of Life Technologies Corporation.

Liquid

This Alexa Fluor® 647 conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2-8°C Wet Ice

Red Laser (633 nm) Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Mouse Anti-Human p34^cdc2 Serine/Threonine Kinase (Clone 17) recognizes an epitope on Human p34^cdc2 Serine/Threonine Kinase. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

p34^cdc2 is thought to be activated by cyclin through dephosphorylation at tyrosine residues. Activated p34^cdc2 kinase is involved in a number of mitotic events such as nuclear envelope breakdown and chromosome condensation through phosphorylation of serine/threonine residues of substrates such as nuclear lamins, histones, pp60c-src, nucleolin and RNA polymerase II. It is then inactivated by a tyrosine kinase. Anti-p34^cdc2 can be used in western blotting, immunoprecipitation and immunohistochemistry. The antibody precipitates an active kinase.

1. Moreno, S. et al. (1990) Cell 61:549
2. Ward, GE. et al. (1990) Cell 61:561
3. Langan, TA. et al. (1989) Mol. Cell. Biol. 9:3860