Anti-Human PDGF Rβ – Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human PDGF Rβ (Accession # P09619) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831587 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing PDGF Rβ. A suggested method would be to stain cells expressing PDGF Rβ with 0.50 µg per 1 - 2.5 x 105 cells in a total staining volume of ≤200 µl followed by PN:A104. Western Blotting: To detect Human PDGF Rβ this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human PDGF Rβ is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P254. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Platelet-Derived Growth Factor Receptor β (PDGF Rβ) recognizes Human PDGF Rβ. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Platelet-derived growth factor receptor-β (PDGFRβ) is a well-characterized plasma membrane receptor with endogeneous tyrosine kinase activity, which is autophosphorylated in response to binding of dimeric platelet-derived growth factor (PDGF) ligand.1 PDGFRβ mediates signaling important for the proliferation, migration, and survival of mesenchymal cells.2 PDGFRβ is found in all cell types 3 and is activated in breast carcinoma cells and indicate a possible role of the receptor in breast cancerogenesis.4 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Gilliland, GD. et al. (1996) Proc Natl Acad Sci USA 93: 14845 2. Ronnstrand, L. et al. (1998) Biochim Biophys Acta 1378: F79 3. Fischer, B. et al. (1997) Acta Anatomica 159: 194 4. Valius, M. et al. (2005) Biologija 1: 61 Technical Protocols  Certificate of Analysis |
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