Anti-Human PKR (NT)

Pricing & Details

Product No.P229
Protein
PKR
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Protein Kinase RNA-activated, Interferon-inducible RNA-dependent Protein Kinase
Applications
IHC FFPE
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
P229-20ug
20 µg
$195.00
In stock
Max:
Min: 1
Step: 1
P229-0.1 mg
0.1 mg
$397.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
PN:P231
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human PKR recognizes an epitope near the N-terminus of human, mouse and rat PKR. This polyclonal antibody was purified using affinity chromatography.
Background
The interferon-inducible, double-stranded RNA (dsRNA)- dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-α) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs (1) that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis (2) and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity (3,4) in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the nonstructural protein (NS1) of the influenza virus (5). More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis (6).

Antigen Details

Protein
PubMed

References & Citations

1. Galabru, J. et al. (1990) Cell 62 :379-90.
2. Vandenabeele, P. et al. (2001) J. Biol. Chem. 276:41620-8.
3. Gil, J. and Esteban, M. (2000) Apoptosis 5:107-14.
4. Esteban, M. et al. (2002) FEBS Lett. 19:3665-74.
IHC
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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