Anti-Human Serpin E1 (Clone 242816)

Anti-Human Serpin E1 (Clone 242816)

Product No.: S606

[product_table name="All Top" skus="S606"]

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Clone
242816
Target
Serpin E1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Plasminogen Activator Inhibitor-1, Serpin E1, PAI-1
Isotype
IgG1
Applications
ELISA Cap
,
N
,
WB

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Product Size

Data

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human Serpin E1 (Accession # P05121)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml.
Western Blotting: To detect Human Serpin E1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Serpin E1 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P301.
Additional Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, #1786-PI) inhibition of Recombinant Human u‑Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, #1310-SE) cleavage of the fluorogenic peptide substrate Z-GGR-AMC (100 µM). The Neutralization Dose (ND50) is typically 0.3 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Serpin E1 (Clone 242816) recognizes an epitope on Human Serpin E1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Details

PubMed
NCBI Gene Bank ID
Elisa Sandwich Protocol
N
General Western Blot Protocol
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Formats Available

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Prod No.
Description
S606
Products are for research use only. Not for use in diagnostic or therapeutic procedures.