Anti-Human ST2/IL-1 R4

Sf 21 - Derived Recombinant Human ST2

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

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Immunohistochemistry: Suitable for use at concentration of 1-15 µg/mL.
Neutralization: Measured by its ability to neutralize IL‑33-induced IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC). Blom, L and Poulsen, LK (2012) JI 189:4331. The Neutralization Dose (ND50) is typically 0.1-0.6 μg/mL in the presence of 1 ng/mL Recombinant Human IL‑33.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Goat Anti-Human ST2 recognizes Human ST2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

ST2 belongs to the immunoglobulin superfamily and bears significant amino acid identity (~25%) to the extracellular portion of both type I and type II interleukin 1 receptors (IL-1R). Some of the intracellular residues that are required for signal transduction through the IL-1R and are conserved in the Drosophila Toll protein are also found in the membrane form of ST2. Furthermore, the gene for ST2 was mapped to mouse chromosome 1 closely linked to the il-1r locus containing both type I and type II receptor genes in support of their common ancestry.