Anti-Human ST2
Anti-Human ST2
Product No.: S582
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Clone 97203 Target ST2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names T1, IL-1 R4, IL-33 R Isotype IgG1 Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Sf 21-Derived Recombinant Human ST2 (Accession # BAA02233) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831791 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human ST2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T263. The detection limit for Human ST2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. ELISA Capture: Suitable for use at concentration of 2-8 µg/mL. in combination with: Detection Reagent: Human/Primate ST2/IL-33R Biotinylated Antibody. Standard: Recombinant Human ST2/IL-33R Fc Chimera Protein, CF. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human ST2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human ST2 (at 5 ng/ml) is 0.2 - 1 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human ST2 (Clone 97203) recognizes an epitope on Human ST2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background ST2 belongs to the immunoglobulin superfamily and bears significant amino acid identity (~25%) to the extracellular portion of both type I and type II interleukin 1 receptors (IL-1R). Some of the intracellular residues that are required for signal transduction through the IL-1R and are conserved in the Drosophila Toll protein are also found in the membrane form of ST2. Furthermore, the gene for ST2 was mapped to mouse chromosome 1 closely linked to the il-1r locus containing both type I and type II receptor genes in support of their common ancestry. PubMed References & CitationsTechnical Protocols N  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
