This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
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Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human Tail-Interacting Protein 47 (TIP47) recognizes an epitope near the C-terminus of Human and Rat TIP47. This polyclonal antibody was purified using affinity chromatography.
Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.
References & Citations
1. Barbero, P. et al. (2002) J. Cell Biol. 156:511
2. Carroll, KS. et al. (2001) Science 292:1373
3. Aivazian, D. et al. (2006) J. Cell Biol. 173:917
4. Ganley, IG. et al. (2004) Mol. Biol. Cell 15:5420
Products are for research use only. Not for use in diagnostic or therapeutic procedures.