Anti-Human ZIPK (Intermediate Domain) (Previously QED Part No.: 2431)

PN:Z101

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

This antibody is also fully validated for immunocytochemistry (ICC) and immunofluorescence (IF) assays. For both applications, an initial starting concentration of 5 μg/mL is recommended, with optimal dilutions to be determined by the investigator.

Rabbit Anti-Human ZIPK recognizes an epitope in the intermediate domain of Human, Mouse and Rat ZIPK. This polyclonal antibody was purified using affinity chromatography.

Biological Role of ZIP Kinase (ZIPK / DAPK3) in Apoptosis

Programmed cell death, or apoptosis, is a tightly regulated cellular pathway driven by death domain-containing adapter molecules, the caspase protease family, and specific signaling enzymes. Upstream serine/threonine protein kinases, such as ASK-1 and RIP, are well-characterized mediators of these apoptotic cascades.

ZIP kinase (ZIPK)**—also widely known as Death-Associated Protein Kinase 3 (DAPK3) or Dlk—is a vital serine/threonine kinase that directly regulates apoptosis and cell survival. Structurally, ZIP kinase features an N-terminal catalytic domain and a unique C-terminal leucine zipper motif. This leucine zipper structure allows ZIPK to bind to ATF4, an important member of the ATF/CREB transcription factor family.

Kinase Subfamily Homology and Tissue Expression
ZIP kinase shares high sequence homology within its catalytic domain with DAP kinase (Death-Associated Protein Kinase), a known mediator of interferon-gamma ($\gamma$-interferon) induced cell death. Together, the ZIP and DAP kinase groups represent a specialized protein kinase subfamily that orchestrates apoptotic signaling and cellular remodeling through their catalytic activities.

While the overexpression of ZIP kinase actively triggers apoptosis, its messenger RNA (mRNA) is ubiquitously expressed across a wide range of mammalian tissues. Investigating ZIPK/DAPK3 regulation and its downstream targets is a major focus in cancer biology, tumor suppressor research, and cell signaling studies.

1. Kawai, T. et al. (1998) Mol. Cell Biol. 18:1642