Anti-Lactoferrin Antibody (21101)

Anti-Lactoferrin Antibody (21101)

Product No.: 21101

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Clone
2B8
Target
Lactoferrin
Formats AvailableView All
Product Type
Monoclonal
Alternate Names
Lactoferrin, EC 3.4.21.-, Growth-inhibiting protein 12, Talalactoferrin [Cleaved into: Lactoferricin-H
Isotype
Mouse IgG1
Applications
WB,ELISA

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified lactoferrin from human milk.
Product Concentration
Lot Specific
Formulation
PBS containing 0.1% sodium azide
State of Matter
Liquid
Product Preparation
Purified by Protein A affinity chromatography
Storage and Handling
These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
This antibody may be used in immuno-assays to detect and quantitate human lactoferrin. Not all applications have been investigated.
Additional Applications Reported In Literature ?
ELISA,
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This antibody reacts in Western blots of whole serum proteins with a lacto- ferrin band of 77kD. No cross-reactivity with other human serum proteins.
Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. {PubMed:22900286}.; [Lactotransferrin]: Major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions (PubMed:14573629, PubMed:1599934, PubMed:6802759, PubMed:3169987, PubMed:11179314, PubMed:12693969). Has antimicrobial activity, which depends on the extracellular cation concentration (PubMed:6802759). Antimicrobial properties include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane (PubMed:14573629, PubMed:1599934, PubMed:6802759, PubMed:3169987, PubMed:11179314, PubMed:12693969). Can also prevent bacterial biofilm development in P.aeruginosa infection (PubMed:12037568). Has weak antifungal activity against C.albicans (PubMed:11083624). Has anabolic, differentiating and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth (PubMed:15166119). Promotes binding of species C adenoviruses to epithelial cells, promoting adenovirus infection (PubMed:17079302). Can inhibit papillomavirus infections (PubMed:17481742). Stimulates the TLR4 signaling pathway leading to NF-kappa-B activation and subsequent pro-inflammatory cytokine production while also interfering with the lipopolysaccharide (LPS)-stimulated TLR4 signaling (PubMed:20345905). Inhibits neutrophil granulocyte migration to sites of apoptosis, when secreted by apoptotic cells (PubMed:19033648). Stimulates VEGFA-mediated endothelial cell migration and proliferation (PubMed:16842782). Binds heparin, chondroitin sulfate and possibly other glycosaminoglycans (GAGs) (PubMed:9359845). Also binds specifically to pneumococcal surface protein A (PspA), the lipid A portion of bacterial lipopolysaccharide (LPS), lysozyme and DNA (PubMed:9359845). {PubMed:11083624, PubMed:11179314, PubMed:12037568, PubMed:12693969, PubMed:14573629, PubMed:15166119, PubMed:1599934, PubMed:16842782, PubMed:17079302, PubMed:17481742, PubMed:19033648, PubMed:20345905, PubMed:3169987, PubMed:6802759, PubMed:9359845}.; Lactoferricin binds to the bacterial surface and is crucial for the bactericidal functions. Has some antiviral activity against papillomavirus infection (PubMed:17481742). N-terminal region shows strong antifungal activity against C.albicans (PubMed:11083624). Contains two BBXB heparin-binding consensus sequences that appear to form the predominate functional GAG-binding site. {PubMed:11083624, PubMed:17481742}.; [Kaliocin-1]: Has antimicrobial activity and is able to permeabilize different ions through liposomal membranes. {PubMed:12693969}.; [Lactoferroxin-A]: Has opioid antagonist activity (PubMed:1369293). Shows preference for mu-receptor (PubMed:1369293). {PubMed:1369293}.; [Lactoferroxin-B]: Has opioid antagonist activity (PubMed:1369293). Shows higher degrees of preference for kappa-receptors than for mu-receptors (PubMed:1369293). {PubMed:1369293}.; [Lactoferroxin-C]: Has opioid antagonist activity (PubMed:1369293). Shows higher degrees of preference for kappa-receptors than for mu-receptors (PubMed:1369293). {PubMed:1369293}.; The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions (PubMed:12535064). This function contributes to the antimicrobial activity (PubMed:12535064). Shows a preferential cleavage at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin sites (PubMed:12535064). {PubMed:12535064}.; [Isoform DeltaLf]: Transcription factor with antiproliferative properties and ability to induce cell cycle arrest (PubMed:15222485). Binds to the DeltaLf response element found in the SKP1, BAX, DCPS, and SELENOH promoters (PubMed:22320386). {PubMed:15222485, PubMed:22320386}.
NCBI Gene Bank ID
UniProt.org
Research Area
Hormones/Proteins

References & Citations

Formats Available

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Prod No.
Description
21101
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.