Anti-LbCpf1 (Cas12a) [Clone 2D5-6G11] – Purified No Carrier Protein

His-tagged recombinant fragment from the N-terminal region of Lachnospiracea bacteria

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Purified antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

2-8C

Anti-LbCpf1 (Cas12a)

CRISPR-associated protein 12a (Cas12a), also known as LbCpf1, is a 144kDa class II, type V CRISPR-associated endonuclease. Originating in bacteria, Cas12a targets and destroys viral genomes and other foreign genetic material by assembling a ribonucleoprotein complex of a CRISPR RNA (crRNA) and Cas12a (1). The specificity of the crRNA targeting makes for a useful biotechnology tool; the crRNA sequence can be modified to direct Cas12a to a specific DNA sequence for targeted DNA editing (2). Following recognition of a thymine-rich protospacer adjacent motif (PAM), Cas12a generates staggered cuts in the DNA, creating ‘sticky’ ends (2).

The hybridoma 2D5-6G11 was developed using a recombinant fragment from the N-terminal region of Cas12a in Lachnospiracea bacteria. 2D5-6G11 has been used in WB, IF, and FC applications.

A0A5S8WF58

1. Paul, B., & Montoya, G. (2020). CRISPR-Cas12a: Functional overview and applications. Biomedical journal, 43(1), 8-17.

2. Yang, H., Gao, P., Rajashankar, K. R., & Patel, D. J. (2016). PAM-dependent target DNA recognition and cleavage by C2c1 CRISPR-Cas endonuclease. Cell, 167(7), 1814-1828.