Anti-Mouse Angiopoietin3 (Clone 113504)

Anti-Mouse Angiopoietin-3 (Clone 113504)

Product No.: A382


Clone 113504 Target Angiopoietin-3 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Angiopoietin-Like-1 (ANGPTL1), ARP1, ANGPT3, AngY, KIAA0351, UNQ162, dJ595C2 Isotype IgG2b Applications WB


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Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen E. coli-Derived Recombinant Mouse Angiopoietin-3 Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828130 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse ANG-3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse ANG-3 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Rat Anti-Mouse Angiopoietin-3 (ANG-3) (Clone 113504) recognizes an epitope on Mouse ANG-3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Angiopoietins 3 (Ang-3) is a secretory protein¹ and a ligands of Tie-2 receptor tyrosine kinase.² Ang3 is most abundant in adrenal gland, placenta, thyroid gland, heart and small intestine.¹ It inhibits endothelial cell proliferation and survival and blocks Ang-1.² PubMed Angiopoietin-3 NCBI Gene Bank ID 51378 UniProt.org Information on Uniprot.org References & Citations 1. Koh, GY. et al. (1999) FEBS Lett. 443: 353 2. Yu, Q. et al. (2004) Cancer Res. 64: 6119 View product citations for antibody A382 on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

E. coli-Derived Recombinant Mouse Angiopoietin-3

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828130

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse ANG-3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse ANG-3 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse Angiopoietin-3 (ANG-3) (Clone 113504) recognizes an epitope on Mouse ANG-3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Angiopoietins 3 (Ang-3) is a secretory protein¹ and a ligands of Tie-2 receptor tyrosine kinase.² Ang3 is most abundant in adrenal gland, placenta, thyroid gland, heart and small intestine.¹ It inhibits endothelial cell proliferation and survival and blocks Ang-1.²

PubMed

Angiopoietin-3

NCBI Gene Bank ID

51378

UniProt.org

Information on Uniprot.org

References & Citations

1. Koh, GY. et al. (1999) FEBS Lett. 443: 353
2. Yu, Q. et al. (2004) Cancer Res. 64: 6119

View product citations for antibody A382 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
A382	Anti-Mouse Angiopoietin-3 (Clone 113504)

Formats Available

Prod No.	Description
A382	Anti-Mouse Angiopoietin-3 (Clone 113504)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Mouse Angiopoietin-3 (Clone 113504)