E. coli-Derived Recombinant Mouse Angiopoietin-3
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse ANG-3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse ANG-3 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rat Anti-Mouse Angiopoietin-3 (ANG-3) (Clone 113504) recognizes an epitope on Mouse ANG-3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Angiopoietins 3 (Ang-3) is a secretory protein1 and a ligands of Tie-2 receptor tyrosine kinase.2 Ang3 is most abundant in adrenal gland, placenta, thyroid gland, heart and small intestine.1 It inhibits endothelial cell proliferation and survival and blocks Ang-1.2
NCBI Gene Bank ID
References & Citations
1. Koh, GY. et al. (1999) FEBS Lett. 443: 353
2. Yu, Q. et al. (2004) Cancer Res. 64: 6119
Products are for research use only. Not for use in diagnostic or therapeutic procedures.