Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Rat Immunogen E. coli-Derived Recombinant Mouse Angiopoietin-3 Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828130 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse ANG-3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse ANG-3 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse Angiopoietin-3 (ANG-3) (Clone 113504) recognizes an epitope on Mouse ANG-3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Angiopoietins 3 (Ang-3) is a secretory protein1 and a ligands of Tie-2 receptor tyrosine kinase.2 Ang3 is most abundant in adrenal gland, placenta, thyroid gland, heart and small intestine.1 It inhibits endothelial cell proliferation and survival and blocks Ang-1.2 Antigen DetailsProtein PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Koh, GY. et al. (1999) FEBS Lett. 443: 353 2. Yu, Q. et al. (2004) Cancer Res. 64: 6119 Technical Protocols |
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