Anti-Mouse CD107b (LAMP-2/Mac-3) (Clone M3/84) – Biotin

Surface glycoproteins from macrophage membranes of peritoneal exudate cells of C57BL/6 mice

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

M3/84 activity is directed against mouse CD107b (Mac-3), also known as LAMP-2.

Macrophages function in phagocytosis of foreign organisms and materials as well as resistance to tumors¹. Additionally, they play roles in T lymphocyte immune responsiveness, inflammatory responses, and regulation of hematopoiesis. Lysosomes are the membrane-bound vacuoles that digest foreign materials and perform specialized autolytic functions². Lysosomes also play a role in antigen presentation, cholesterol metabolism, and hydrolysis of exogenous materials³ .

CD107b (LAMP-2, Mac-3) is a member of the lysosome-associated membrane protein (LAMP) family and is an important component of the lysosomal membrane involved in autophagy^3,4 . CD107b is a highly glycosylated transmembrane protein, with glycosylation being necessary for its stability^5,6. Mice deficient in CD107b have a severe phenotype, with 50% dying aged 20-40 days⁶. In humans, mutations in CD107b lead to Danon disease.

The immunogen used to generate M3/84 was produced by first purifying surface glycoproteins from macrophage membranes of peritoneal exudate cells of C57BL/6 mice⁵. Next, previously recognized antigens were removed by immunoadsorbent columns to obtain a glycoprotein- enriched fraction. Then, (Lewis x BN) F₁ rats were injected and splenocytes fused to mouse myeloma NS1 for hybridoma production.

CD107b (LAMP-2) is a constituent of the lysosomal membrane. CD107b is present in macrophages, granulocytes, peripheral blood lymphocytes, bronchial epithelium, alveolar lining cells, intestinal epithelium, and some dendritic and endothelium cells.

1 Ho MK, Springer TA. J Biol Chem. 258(1):636-642. 1983.
2 Chen JW, Murphy TL, Willingham MC, et al. J Cell Biol. 101(1):85-95. 1985.
3 Kannan K, Stewart RM, Bounds W, et al. Cell Immunol. 171(1):10-19. 1996.
4 Qiao L, Hu J, Qiu X, et al. Autophagy. 19(11):2837-2852. 2023.
5 Springer TA. J Biol Chem. 256(8):3833-3839. 1981.
6 Eskelinen EL. Mol Aspects Med. 27(5-6):495-502. 2006.
7 Flotte TJ, Springer TA, Thorbecke GJ. Am J Pathol. 111(1):112-124. 1983.
8 Knisley KA, Weitlauf HM. J Reprod Fertil. 97(2):521-527. 1993.