Anti-Mouse CD107b (LAMP-2/Mac-3) (Clone M3/84) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Clone M3/84 has been validated for use on PhenoCycler® using [Mouse/Human] [Tissue] [Tissue Type either FFPE or FF] tissue.

Surface glycoproteins from macrophage membranes of peritoneal exudate cells of C57BL/6 mice

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.

This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

M3/84 activity is directed against mouse CD107b (Mac-3), also known as LAMP-2.

CD107b (LAMP-2) is a constituent of the lysosomal membrane. CD107b is present in macrophages, granulocytes, peripheral blood lymphocytes, bronchial epithelium, alveolar lining cells, intestinal epithelium, and some dendritic and endothelium cells.

Macrophages function in phagocytosis of foreign organisms and materials as well as resistance to tumors¹. Additionally, they play roles in T lymphocyte immune responsiveness, inflammatory responses, and regulation of hematopoiesis. Lysosomes are the membrane-bound vacuoles that digest foreign materials and perform specialized autolytic functions². Lysosomes also play a role in antigen presentation, cholesterol metabolism, and hydrolysis of exogenous materials³ .

CD107b (LAMP-2, Mac-3) is a member of the lysosome-associated membrane protein (LAMP) family and is an important component of the lysosomal membrane involved in autophagy^3,4 . CD107b is a highly glycosylated transmembrane protein, with glycosylation being necessary for its stability^5,6. Mice deficient in CD107b have a severe phenotype, with 50% dying aged 20-40 days⁶. In humans, mutations in CD107b lead to Danon disease.

The immunogen used to generate M3/84 was produced by first purifying surface glycoproteins from macrophage membranes of peritoneal exudate cells of C57BL/6 mice⁵. Next, previously recognized antigens were removed by immunoadsorbent columns to obtain a glycoprotein- enriched fraction. Then, (Lewis x BN) F₁ rats were injected and splenocytes fused to mouse myeloma NS1 for hybridoma production.

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2 Chen JW, Murphy TL, Willingham MC, et al. J Cell Biol. 101(1):85-95. 1985.
3 Kannan K, Stewart RM, Bounds W, et al. Cell Immunol. 171(1):10-19. 1996.
4 Qiao L, Hu J, Qiu X, et al. Autophagy. 19(11):2837-2852. 2023.
5 Springer TA. J Biol Chem. 256(8):3833-3839. 1981.
6 Eskelinen EL. Mol Aspects Med. 27(5-6):495-502. 2006.
7 Flotte TJ, Springer TA, Thorbecke GJ. Am J Pathol. 111(1):112-124. 1983.
8 Knisley KA, Weitlauf HM. J Reprod Fertil. 97(2):521-527. 1993.