Anti-Mouse CD11a (Clone FD441.8) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD11a (Clone FD441.8) – Purified in vivo GOLD™ Functional Grade
Product No.: C303
Clone FD441.8 Target CD11a Formats AvailableView All Product Type Monoclonal Antibody Alternate Names LFA-1α Isotype Rat IgG2b Applications FA , FC , N , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Cloned CTL B18 cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737475 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this FD441.8 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone FD441.8 recognizes an epitope on mouse CD11a. Background LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis. Antigen Distribution CD11a is present on thymocytes, blood lymphocytes, bone marrow cells and certain lymphoma and macrophage-like cell lines. Ligand/Receptor ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50) Function LFA-1 plays a central role in leukocyte intercellular adhesion. PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Costimulatory Molecules . Immunology . Signal Transduction Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone FD441.8 is a rat monoclonal antibody targeting mouse CD11a (LFA-1α) and is widely used in vivo to functionally block or deplete LFA-1 activity, most commonly to inhibit leukocyte adhesion, migration, and T cell activation in murine models. Key in vivo applications in mice include:
Additional standard uses:
Less common or indirect applications:
Experimental considerations: Successful use in vivo depends on optimizing dose, timing, and route of administration for each experimental design, and using functionally validated, low-endotoxin antibody preparations. Summary Table: Common In Vivo Applications of Clone FD441.8 in Mice
Note: While clone FD441.8 is validated for in vivo functional inhibition, depletion of cells expressing CD11a is not a common or efficient application due to its mode of action as a blocking rather than depleting antibody. For depleting certain leukocyte subsets, depletion-specific clones or toxin-conjugated antibodies are preferable. References marking the main points: Based on the search results, FD441.8 (an anti-mouse CD11a/LFA-1α antibody) is commonly used in combination with several other antibodies and proteins in research applications: Co-stimulation Blockade StudiesFD441.8 is frequently paired with anti-CD154 monoclonal antibodies in co-stimulation blockade protocols. In pancreatic islet xenograft survival studies, researchers used an equal mixture of FD441.8 and MR-1 (an anti-CD154 antibody) to achieve subtherapeutic co-stimulation blockade. This combination targets different aspects of the immune response by blocking both LFA-1-mediated adhesion and CD154-dependent costimulatory signals. Lymphocyte Function StudiesIn lymphocyte homing and trafficking research, FD441.8 has been used alongside other adhesion molecule antibodies including anti-CD4 (clone GK1.5), anti-CD8 (clone 53-6.7.2), and anti-α4 (clone PS/2). This combination allows researchers to comprehensively examine the roles of different integrins and surface markers in lymphocyte migration to specific tissues like bronchus-associated lymphoid tissue. Natural Pairing with LigandsGiven that FD441.8 targets LFA-1α (CD11a), it naturally functions in systems involving LFA-1's binding partners. The antibody is relevant in studies examining interactions with ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50), which are the natural ligands for LFA-1. Additionally, since CD11a combines with CD18 (integrin beta-2 chain) to form the complete LFA-1 heterodimer, studies may examine both components together. Clone FD441.8 is a monoclonal antibody that targets mouse CD11a, also known as LFA-1α, which plays a crucial role in cell adhesion and immune responses. Here are the key findings from its citations in scientific literature: Key Findings
ConclusionClone FD441.8 is a valuable tool in immunological research, particularly in studies involving LFA-1 and its role in immune cell adhesion and trafficking. Its applications range from basic research to potential therapeutic interventions, where modulating immune responses is essential. Dosing regimens of clone FD441.8 (anti-LFA-1) in mouse models typically involve intraperitoneal injections of the antibody at a dose of 250 μg per mouse on specific days post-transplant (days 0, 2, 4, and 6), followed by biweekly injections until 3 months post-transplantation. Most published protocols use this antibody as part of multi-agent immune suppression to enable transplantation success, particularly in strains resistant to xenograft acceptance, such as C57BL/6 mice.
Regimen variations may depend on:
Additional relevant details:
In summary, while FD441.8 dosing in mouse models is usually 250 μg/mouse i.p. given repeatedly over several weeks to months, variations reflect study-specific goals, mouse strain immunogenicity, and combination with other immunosuppressive agents. References & Citations1. Suratt, Benjamin T. et al. (2009) J Immunol.182(1): 604–612. PubMed 2. Wuthrich, R. P. et al. (1991) Cell Immunol. 144:22 3. Sanchez-Madrid, F. et al. (1983) J. Exp. Med. 158(20):586 4. Pont, S. et al. (1986) J. Immunol. 136(10):3750 Technical ProtocolsCertificate of Analysis |
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