Anti-Mouse CD11a (Clone FD441.8) – Purified in vivo GOLD™ Functional Grade

The FD441.8 clone is primarily used in in vivo mouse studies for LFA-1 neutralization and immune system modulation experiments. This monoclonal antibody targets mouse LFA-1? (CD11a), which is a critical component of the immune system's adhesion and signaling mechanisms.

Primary Applications in In Vivo Studies

The FD441.8 antibody is specifically designed for in vivo LFA-1 neutralization. By blocking LFA-1 function, researchers can study the role of this integrin in various immune processes and disease models. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50), and also functions in lymphocyte costimulatory signaling.

Mechanism of Action

When administered in vivo, the FD441.8 antibody binds to the LFA-1? chain (CD11a) on the surface of leukocytes, including lymphocytes, monocytes, macrophages, and granulocytes. This binding effectively neutralizes LFA-1 function, disrupting:

• Cell adhesion processes that are crucial for immune cell trafficking
• Intercellular interactions between immune cells
• Costimulatory signaling pathways in lymphocytes

Research Applications

The neutralization of LFA-1 using FD441.8 allows researchers to investigate:

• Immune cell migration and trafficking patterns
• Inflammatory responses and their regulation
• T cell activation and costimulatory mechanisms
• Leukocyte-endothelial interactions during inflammation
• Natural killer cell cytotoxicity processes

Formulation and Quality Standards

For in vivo use, the FD441.8 antibody is formulated in PBS at pH 7.0 without stabilizers or preservatives. It meets stringent quality requirements including low endotoxin levels (<2EU/mg), high purity (>95%), and sterility through 0.2 ?m filtration. The antibody is produced in an animal-free facility and purified using Protein G, ensuring consistent performance in experimental settings.

This clone has become a standard tool for researchers studying immune system function, particularly in models where blocking cell adhesion and immune cell interactions is necessary to understand disease mechanisms or test therapeutic interventions.

Based on the search results, the sterile packaged clone FD441.8 (Biotin Anti-Mouse CD11a Antibody) should be stored at 2-8°C for up to 12 months. The storage instructions specifically note that the product should not be frozen.

This temperature range of 2-8°C is equivalent to standard refrigeration conditions, which maintains the stability and sterility of the antibody clone while preventing degradation that could occur at higher temperatures or damage from freezing.

When considering the use of the FD441.8 monoclonal antibody targeting CD11a, several other antibodies and proteins are commonly used in immunological research, often in conjunction with or in similar contexts to FD441.8. Here are a few examples:

1. Anti-CD18 Antibodies: These are often used alongside CD11a antibodies because CD11a and CD18 form the LFA-1 complex, which is crucial for leukocyte adhesion .

2. Anti-ICAM-1 (CD54) Antibodies: Since ICAM-1 is a ligand for LFA-1, studying its interaction with CD11a is important for understanding immune responses and cell adhesion .

3. Anti-CD3 or CD4/CD8 Antibodies: These are used to identify specific T cell populations, which are targets of interest in immunological studies involving CD11a .

4. Phage Display-Derived Antibodies: While not directly used with FD441.8, these antibodies (e.g., those targeting IGF-1R, ErbB3, TRAIL-R2) are used in broader immunological research contexts, including cancer and autoimmune diseases .

These antibodies can be used in various applications, including flow cytometry, immunofluorescence, and in vivo studies, to explore different aspects of immune cell function and interactions.

There are no direct key findings cited in the scientific literature for "clone FD441.8" as a subject of research per se; rather, the available references mention FD441.8 as a technical reagent (a blocking anti–LFA-1 antibody) used in experimental protocols. Here’s what the literature reveals:

• FD441.8 is described as a blocking anti–LFA-1 (lymphocyte function–associated antigen-1) antibody, obtained from Bio X Cell, and used in immunological studies to inhibit LFA-1-mediated cell interactions. However, the cited paper does not report any biological discoveries or mechanistic insights specifically about clone FD441.8 itself; it is used as a tool to modulate immune cell behavior in the context of adoptive T cell transfer experiments.
• A second citation uses FD441.8 in an allograft model to antagonize LFA-1, but again, the focus is on the effect of LFA-1 blockade on graft-infiltrating leukocytes, not on the properties or specific findings related to clone FD441.8 itself. The dose of FD441.8 was chosen based on preliminary allograft survival data, but no novel biology or unique features of FD441.8 as a clone are discussed.
• No primary research articles were found that focus on clone FD441.8 as a central subject (e.g., characterizing its binding, specificity, structure, or unique functional properties independent of its use as an experimental reagent).

Summary Table

Conclusion

Clone FD441.8 is referenced in the scientific literature solely as a technical reagent—a monoclonal antibody used to block LFA-1 in immunological experiments—not as the focus of any key scientific findings. No unique biological insights or discoveries about FD441.8 itself are reported in the cited articles; it is valued for its role in modulating LFA-1-dependent processes in experimental systems.