Anti-Mouse CD134 (Clone OX-86) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C856
Clone
OX-86
Protein
CD134
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
OX-40, TNFSF4
Isotype
Rat
IgG1 κ
Applications
Act
,
FC
,
IHC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C856-1.0mg
1.0 mg
$225.00
In stock
Max:
Min: 1
Step: 1
C856-5.0mg
5.0 mg
$445.00
In stock
Max:
Min: 1
Step: 1
C856-25mg
25 mg
$1,285.00
In stock
Max:
Min: 1
Step: 1
C856-50mg
50 mg
$2,045.00
In stock
Max:
Min: 1
Step: 1
C856-100mg
100 mg
$2,800.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Other Applications Reported In Literature ?
FC
IHC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone OX-86 reacts with murine CD134 (OX-40, TNFSF4).
Antigen Distribution
CD134 is expressed on activated CD4 and CD8 T cells, activated regulatory T cells, B cells, NKT cells, NK cells, and neutrophils.
Background
CD134 functions as an important immune checkpoint, and its depletion in murine mouse models demonstrate that lack of CD134 expression leads to reduced CD4+ and CD8+ T cells1. When CD134 is bound by its corresponding ligand (OX-40L), an optimal T cell response is generated and plays a significant role in determining the amount of memory T-cells remaining after the immune response1. CD134 has also been found to play an important role in carcinogenesis, as treatment with activating in vivo antibodies against CD134 enhanced tumor growth, suggesting that CD134 is an important tumor suppressor, and its absence disrupts the immune response to tumors2,3.

Antigen Details

Protein
PubMed
Research Area
Immunology

References & Citations

1. Redmond WL, Ruby CE, Weinberg AD. Crit Rev Immunol. 29(3):187-201. 2019
2. Morris A, Vetto JT, Ramstad T, et. al. Breast Cancer Res Treat. 67: 71–80. 2001.
3. Weinberg AD, Rivera MM, Prell R, et. al. J Immunol. 164: 2160–9. 2000.
4. al-Shamkhani A, Birkeland ML, Puklavec M, et. al. Eur J Immunol. Aug;26(8):1695-9. 1996.
5. Higgins LM, McDonald SA, Whittle N, et. al. J Immunol. Jan 1;162(1):486-93. 1999.
Flow Cytometry
IHC
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.