Anti-Mouse CD153 – Biotin

CHO cells stably transfected with murine CD153

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

RM153 activity is directed against murine CD153.

CD153, also known as CD30 ligand or CD30-L, is a cytokine member of the TNF superfamily that interacts with CD30 (TNFRSF8) in T cell proliferation¹. CD153 dysregulation is found in some cancers, inflammatory and autoimmune diseases. CD153/CD30 interactions also play a role in the T cell-dependent anti-mycobacterial immune response². Higher bacterial burdens are observed when either CD153 or CD30 are blocked or deficient. CD30/CD153 interactions are therefore a therapeutic target.

RM153 was generated by immunizing Sprague Dawley rats with CHO cells stably transfected with murine CD153³. Splenocytes were fused with P3U1 myeloma cells. RM153 inhibits the binding of CD30 to CD153. RM153 binds to CD153/CHO, CD153/L5178Y, and CD153/P815 cells but not parental CHO, L5178Y, or P815 cells.

RM153 has been used as a blocking agent in the study of diabetes⁴, allograft models⁵, infectious disease⁶, and atherosclerosis⁷. RM153 blocks interaction between CD30⁺ and CD153⁺ cells and can also deplete CD153⁺ cells 5 . RM153 also inhibits the proliferation of αCD3/CD28 activated T cells⁷.

CD153 is expressed transiently on murine-activated T cells. CD153 is expressed on both Th1 and Th2 cells after anti-CD3 stimulation. CD153 is expressed on CD4⁺ and CD8⁺ T cells in the spleen and pancreatic lymph nodes of young NOD mice.

CD30 (TNFRSF8)

1 Oflazoglu E, Grewal IS, Gerber H. Adv Exp Med Biol. 647:174-185. 2009.
2 Marín ND, García LF. Tuberculosis (Edinb). 102:8-15. 2017.
3 Shimozato O, Takeda K, Yagita H, et al. Biochem Biophys Res Commun. 256(3):519-526.1999.
4 Chakrabarty S, Nagata M, Yasuda H, et al. Clin Exp Immunol. 2003 Sep;133(3):318-325. 2003.
5 Kennedy MK, Willis CR, Armitage RJ. Immunology. 118(2):143-152. 2006.
6 Flórido M, Borges M, Yagita H, et al. J Leukoc Biol. 76(5):1039-1046. 2004.
7 Foks AC, Bot I, Frodermann V, et al. Arterioscler Thromb Vasc Biol. 32(12):2862-2868. 2012.