Anti-Mouse CD18 – APC

Anti-Mouse CD18 – APC

Product No.: C342

[product_table name="All Top" skus="C342"]

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Clone
C71/16
Target
CD18
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
LFA-1, Integrin β2 Chain
Isotype
IgG2a
Applications
FC

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Product Concentration
0.2 mg/ml
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Red Laser (650 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this C71/16 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone C71/16 recognizes an epitope on mouse CD18.
Antigen Distribution
CD18 is present on mouse T-cells, B-cells, NK cells, monocytes, macrophages and granulocytes.
Background
CD18 is a 95kD type I transmembrane glycoprotein. CD18 is the Integrin β2 chain and forms multiple heterodimers with the three integrin α chains, CD11a, CD11b, and CD11c. CD18 binds with CD11a to form LFA-1. LFA-1 is involved in adhesion and binding to antigen presenting cells via interactions with ICAM-1. CD18 binds with CD11b and CD11c forming complement receptors , such as MAC-1, that participate in the innate immune response by recognizing foreign antigen peptides and destroying them via phagocytosis. In humans, lack of functional CD18 causes Leukocyte Adhesion Deficiency (LAD) which is characterized by the inability of circulating leukocytes to respond to foreign bodies, reducing the ability of the immune system to fight off infection.

Antigen Details

Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
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Neuroinflammation
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Neuroscience

References & Citations

Springer, T. et al. (1978) Eur. J. Immunol. 8:539
Flow Cytometry

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.