Anti-Mouse CD18 [Clone C71/16] — Alexa Fluor® 647
Anti-Mouse CD18 [Clone C71/16] — Alexa Fluor® 647
Product No.: C1790AF
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Clone C71/16 Target CD18 Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names Integrin beta-2, Mac-1 beta, macrophage antigen-1 beta, complement receptor C3 subunit beta, lymphocyte function associated antigen 1, cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, 2E6, LAD, Lfa1, MF17, LCAMB, AI528527 Isotype Rat IgG2a κ Applications FC |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse CD18 (>98%) Product Concentration 0.2 mg/ml Formulation This Alexa Fluor® 647 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Alexa Fluor® is a trademark of Life Technologies Corporation. State of Matter Liquid Storage and Handling This Alexa Fluor® 647 conjugate is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only Country of Origin USA Shipping 2-8°C Wet Ice Excitation Laser Red Laser (633 nm) Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this C71/16 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone C71/16 recognizes an epitope on mouse CD18. Background CD18 is a 95kD type I transmembrane glycoprotein. CD18 is the Integrin β2 chain and forms multiple heterodimers with the three integrin α chains, CD11a, CD11b, and CD11c. CD18 binds with CD11a to form LFA-1. LFA-1 is involved in adhesion and binding to antigen presenting cells via interactions with ICAM-1. CD18 binds with CD11b and CD11c forming complement receptors , such as MAC-1, that participate in the innate immune response by recognizing foreign antigen peptides and destroying them via phagocytosis. In humans, lack of functional CD18 causes Leukocyte Adhesion Deficiency (LAD) which is characterized by the inability of circulating leukocytes to respond to foreign bodies, reducing the ability of the immune system to fight off infection. Antigen Distribution CD18 is present on mouse T-cells, B-cells, NK cells, monocytes, macrophages and granulocytes. Ligand/Receptor ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Neuroinflammation . Neuroscience . Complement References & Citations1. Springer, T. et al. (1978) Eur. J. Immunol. 8:539 Technical Protocols Certificate of Analysis |
Formats Available
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Prod No. | Description |
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C1794 | |
C231 | |
C234 | |
C232 | |
C233 | |
C342 | |
C1786 | |
C1787 | |
C1788 | |
C1790 | |
C378 | |
C1790AF | |
C1786AF | |
C678 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
