Anti-Mouse CD1d (CD1.1) – Purified in vivo GOLD™ Functional Grade

The clone 20H2 is a monoclonal antibody used in in vivo mouse studies to specifically target mouse CD1d (CD1.1), a non-classical MHC class I-like molecule expressed on antigen-presenting cells.

In vivo applications of clone 20H2 include:

• Blocking or detection: 20H2 is most commonly used to block or detect CD1d molecules on the surface of mouse cells during experiments involving immune function, glycolipid antigen presentation, or invariant natural killer T (iNKT) cell activation.
• Functional studies: By binding to mouse CD1d, 20H2 can inhibit the interaction between CD1d and iNKT cells, allowing researchers to study the roles of CD1d-restricted immune responses in processes such as tumor immunity, infection, and autoimmunity.

Typical experimental setups:

• In vivo administration: The antibody is injected into mice to block CD1d-mediated activation of iNKT cells and thus evaluate the impact of iNKT cell activity on immune responses.
• Controls and readouts: After in vivo administration, downstream effects are measured via cytokine production, cell profiling, or survival after tumor/viral challenge, depending on the model system.

The 20H2 antibody is a well-established tool in immunological research for modulating or tracking CD1d function in live mice.

The correct storage temperature for sterile packaged clone 20H2 is at 4°C or lower. This typically means refrigeration to maintain stability and sterility.

Supporting details:

• Multiple references from product information guides, including Sigma-Aldrich and related IHC products, specify storing undiluted antibody clones such as 20H2 at 4°C or lower.
• Another similar antibody clone, 6B9 (associated with Anti-Suv4–20H2), is specifically noted to be stable for 1 year at 2–8°C (the typical refrigerator range).
• General best practices for sterile packaged laboratory antibodies and reagents recommend refrigerated storage (2–8°C) unless stated otherwise by the manufacturer for prolonged stability.

If you have the original product packaging, always confirm with the manufacturer’s label or datasheet, as storage requirements can occasionally vary by formulation. For most sterile, ready-to-use IHC antibody clones, refrigeration (2–8°C, and not frozen unless directed) is required.

Commonly, 20H2 (often referring to the Suv4-20h2 histone methyltransferase or its antibody) is studied or used in conjunction with several other antibodies and proteins. In the literature, the most frequently co-profiled or co-immunoprecipitated proteins include:

• HP1? (Heterochromatin Protein 1 alpha): Frequently assessed alongside Suv4-20h2 due to their physical and functional interactions in heterochromatin structure and maintenance. HP1? binds specifically to the Clamp domain of Suv4-20h2.

• Cohesin Complex Proteins (such as Smc1 and Smc3): Cohesin subunits are often used with Suv4-20h2 antibodies to investigate their interaction in chromatin architecture studies. Co-immunoprecipitation and Western blotting with Smc1, Smc3, and sometimes Suz12 are commonly performed with Suv4-20h2.

• Histone H4 (especially H4K20 methyl states): As Suv4-20h2 directly methylates H4K20, antibodies detecting mono-, di-, or tri-methyl H4K20 are often included in parallel to evaluate methylation changes.

• Viral Proteins NP and PB2 (in infection studies): For example, with influenza infection models, Suv4-20h2 binding partners NP and PB2 are probed using specific antibodies to assess interaction and functional outcomes.

Additional notable markers sometimes included, depending on the context:

• Suz12 (component of the PRC2 complex) as an epigenetic control in some chromatin studies.
• Flag-tag antibodies: Common when using Flag-tagged Suv4-20h2 constructs for immunoprecipitation or Western blot analysis.

Summary Table: Commonly Used Antibodies/Proteins with 20H2

Use of these antibodies and proteins depends on the research context, with a focus on heterochromatin structure, histone modification, chromatin compaction, or virus-host interaction studies.

Clone 20H2 is a rat IgG1 monoclonal antibody specific for mouse CD1d, and several scientific publications have cited its use primarily for flow cytometry and in vivo antibody-mediated depletion or blocking studies, particularly in immunology research involving mice. Key findings from these citations focus on the antibody's utility and associated research outcomes:

• Functional Use in Immunology: Clone 20H2 is mainly used for detecting or modulating mouse CD1d expression. CD1d is important for antigen presentation to NKT cells, making this clone valuable in studies of antitumor immunity, inflammatory diseases, and immune cell profiling in mice.

• Control and Validation: Publications highlight that clone 20H2 often appears as a positive or functional antibody in experimental panels. For control experiments, researchers use isotype controls such as control rat IgG1 to confirm the specificity of staining or antibody-mediated effects.

• Research Findings Impacted by 20H2 Use: Experiments using this antibody have informed mechanisms by which DNA hypomethylation, for example, can silence antitumor immune genes by altering the activity or presence of immune cells identified through CD1d staining with 20H2.

Although in-depth outcome data from all citing papers are not available in the summaries, the cited immunology work demonstrates that clone 20H2 is a well-established tool for dissecting immune functions in mouse models, particularly those relating to CD1d-expressing cells and NKT cell biology.

No evidence from the cited literature suggests significant controversy or negative findings related to the performance of clone 20H2; instead, it consistently serves as a technical backbone in immunological investigations in mice.