Anti-Mouse CD1d (CD1.1) – Purified in vivo GOLD™ Functional Grade

C57BL/6 thymocytes, RBL-CD1.1-transfected rat cell line, and CR1-CD1.1 cells

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

20H2 activity is directed against mouse CD1d (CD1.1) and cross reacts with CD1.2

CD1d is a non-classical major histocompatibility complex (MHC) molecule that presents glycolipid instead of peptide antigens¹ . CD1d is non-MHC-encoded, heavily glycosylated, and a surface protein associated with β2-microglobulin² . CD1d is present on invariant natural killer (iNKT) cells, where it elicits an immune response upon ligation with the T cell receptor and agonist α-galactosylceramide (α-GalCer )¹. iNKT cells activated by α-GalCer secrete cytokines and undergo proliferation. Additionally, α-GalCer can induce anti-tumor immunity. CD1d can also interact with the iNKT agonist β-mannosylceramide (β-ManCer).

20H2 was generated by immunizing male Wistar rats with C57BL/6 thymocytes, boosting three times with a RBL-CD1.1-transfected rat cell line, injecting once with BALB/c thymocytes, and finally injecting with CR1-CD1.1 cells² . The resulting spleen cells were fused with Sp2/0 cells for hybridoma production. 20H2 cross-reacts with CD1.2-transfected cells and binds to an epitope in the vicinity of the CD1d antigen-binding groove.

20H2 blocks T cell recognition of CD1d² and can be used to block β-ManCer and α-GalCer agonistic activity¹ . Blocking with 20H2 may also attenuate non-allergic asthma³ and ameliorate some aspects of neurodegenerative pathology in mouse⁴.

CD1d (CD1.1) is expressed on type I natural killer T cells also known as invariant NKT cells. CD1d is expressed by dendritic cells, macrophages, and B cells, particularly splenic marginal zone B cells.

Similar to MHC class I, associate with ß-microglobulin

1 Qin Y, Oh S, Lim S, et al. Exp Mol Med. 51(10):1-9. 2019.
2 Roark JH, Park SH, Jayawardena J, et al. J Immunol. 160(7):3121-3127. 1998.
3 Iwamura C, Nakayama T. Front Immunol. 9:1942. 2018.
4 Shin JH, Park JY, Shin YH, et al. J Immunol. 188(5):2235-2243. 2012.
5 Zeissig S, Peuker K, Iyer S, et al. Proc Natl Acad Sci U S A. 114(39):10449-10454. 2017.
6 Iba M, Kwon S, Kim C, et al. J Neuroinflammation. 21(1):93. 2024.