Anti-Mouse CD200R [Clone OX-131] — Purified No Carrier Protein

Recombinant mouse CD200R extracellular domain fusion protein.

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

Purified antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

OX131 detects both mouse isoforms of CD200R: CD200R(1) from C57BL/6 mice and CD200R_NOD, also known as CD200R(2), from NOD mice. OX131 also cross-reacts with CD200RLe, which is not present in most mouse strains that contain CD200R. OX131 is therefore specific to CD200R in mouse strains that lack CD200RLe, such as C57BL/6 and BALB/c.

CD200R is the cognate receptor of CD200, and when paired, acts as an inhibitor-type receptor that inhibits the ERK pathway via a phosphotyrosine motif that recruits DOK2 and RasGAP¹. Two CD200R alleles, differing by seven amino acids in the extracellular region, have been identified, with one allele present in NOD mice and the other in C57BL/6 mice. Both alleles are capable of binding CD200. CD200R/CD200 interactions are of interest for cancer therapeutics. CD200 is an immune checkpoint molecule². The CD200/CD200R signaling pathway inhibits natural killer cell activation, cytotoxic T cell functions, and M1-polarized macrophage activity.

OX131 was generated by immunizing DA rats with a recombinant protein consisting of the extracellular region of CD200R combined with rat CD4d3+4 as a tag¹. Hybridomas were created by fusing to the Y3 mouse hybridoma line and screened by a flow cytometry-based binding assay. OX131 blocks CD200 binding to both CD200R isoforms. Additionally, by blocking CD200/CD200R interaction, OX131 prevents inhibition by CD200 in a T cell activation assay, resulting in IL-2 release.

CD200R is expressed on various types of leukocytes, including myeloid cells, macrophages, basophils, mast cells, eosinophils, neutrophils, and some dendritic cells. CD200R is not expressed on naïve T and B cells. T cell activation results in limited induction of CD200R.

CD200 (OX-2) / Receptor: CD200R

1 Akkaya M, Aknin ML, Akkaya B, et al. PLoS One. 8(5):e63325. 2013.
2 Nip C, Wang L, Liu C. Biomedicines. 11(12):3326. 2023.