Anti-Mouse CD3ε – DyLight® 488

H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37

This DyLight^® 488 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

This DyLight^® 488 conjugate is stable when stored at 2-8°C. Do not freeze.

FC The suggested concentration for this 145-2C11 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

IHC (Frozen)
IP
Act
B
WB
ICC
Depletion
IF Staining
IHC (Paraffin)
For specific conjugates of this clone, review literature for suggested application details.

Clone 145-2C11 recognizes an epitope on mouse CD3ε.

CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Peptide antigen/MHC-complex