Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Armenian Hamster Immunogen H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37 Product Concentration 0.5 mg/ml Formulation This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Applications and Recommended Usage Flow Cytometry: This antibody can be used at a concentration of ≤ 0.25 µg per 100 µl per million cells (or per 1.0 x 106 cells in a 100 µl total staining volume). Applications and Recommended Usage ? (Quality Tested by Leinco) FC The suggested concentration for this 145-2C11 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Reported Applications For Relevant Conjugates ? IHC (Frozen) IP Act B WB ICC Depletion IF Staining IHC (Paraffin) For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone 145-2C11 recognizes an epitope on mouse CD3ε. Antigen Distribution CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes. Background CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition. Antigen DetailsProtein CD3ε Ligand/Receptor Peptide antigen/MHC-complex PubMed 145-2C11+CD3ε NCBI Gene Bank ID UniProt.org References & CitationsTechnical Protocols |
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