Antibody DetailsReactivity Species Mouse Host Species Armenian Hamster Immunogen H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37 Product Concentration 0.5 mg/ml Formulation This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze. Applications and Recommended Usage ? (Quality Tested by Leinco) FC The suggested concentration for this 145-2C11 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Applications and Recommended Usage ? (Quality Tested by Leinco) Flow Cytometry: This antibody can be used at a concentration of ≤ 0.25 µg per 100 µl per million cells (or per 1.0 x 106 cells in a 100 µl total staining volume). Additional Reported Applications For Relevant Conjugates IHC (Frozen) IP Act B WB ICC Depletion IF Staining IHC (Paraffin) For specific conjugates of this clone, review literature for suggested application details. DescriptionSpecificity Clone 145-2C11 recognizes an epitope on mouse CD3ε. Antigen Distribution CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes. Background CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition. Antigen DetailsProtein Family CD3ε Ligand/Receptor Peptide antigen/MHC-complex NCBI Gene Bank ID UniProt.org Technical Protocols |
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