Anti-Mouse CD3ε [Clone 500A2] — Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD3ε [Clone 500A2] — Purified in vivo PLATINUM™ Functional Grade
Product No.: C2834
Clone 500A2 Target CD3ε Formats AvailableView All Product Type Monoclonal Antibody Alternate Names T3, CD3 Isotype Syrian Hamster IgG2a κ Applications B , FC , IHC FF , in vivo |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Syrian Hamster Recommended Dilution Buffer Immunogen Mouse T-cell receptor Product Concentration ≥ 5.0 mg/ml Endotoxin Level ≤ 0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829592 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 500A2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IHC (Frozen) The suggested concentration for this 500A2 antibody in IHC staining on frozen tissue is 2.5 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application. B Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 500A2 recognizes an epitope on mouse CD3ε. Background CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition. Antigen Distribution CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes. Ligand/Receptor Peptide antigen/MHC-complex. NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Immunology . Signal Transduction Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone 500A2 is a monoclonal antibody targeting mouse CD3ε and is widely used for in vivo immunological research in mice, particularly to engage or deplete T cells and to modulate the immune response. Common in vivo applications include:
Additional context:
In summary, clone 500A2 is primarily used for in vivo T cell depletion or activation, immune response modulation, immunophenotyping, and as a research tool to interrogate T cell-dependent processes in mouse models. Commonly used antibodies or proteins in the literature alongside 500A2 (anti-mouse CD3ε) include other anti-CD3 clones, as well as antibodies against major lymphocyte surface markers and functional proteins. These are selected to aid in immunophenotyping, T cell activation studies, and multi-color flow cytometry assays. Key examples regularly used with 500A2:
In summary, the most frequent partners for 500A2 in published protocols are other anti-CD3 clones (17A2, 145-2C11), antibodies for CD4, CD8, and other lineage markers (CD14, CD20, CD34), and detection secondaries or functional reagents for cell activation and blocking studies. Overview of Clone 500A2 Anti-Mouse CD3ε AntibodyClone 500A2 is a well-characterized monoclonal antibody raised in Syrian hamster that specifically recognizes mouse CD3ε, a critical component of the T cell receptor (TCR) complex. CD3ε is expressed primarily on mature T cells and NK-T cells, with varying levels on thymocytes depending on differentiation status. The TCR complex, including CD3ε, is essential for T cell signaling and immune function. Key Scientific Findings Involving Clone 500A2Epitope Specificity and Cross-Blocking
Functional and Biological Applications
Technical Validation
Citation Context and Usage
Summary Table
ConclusionClone 500A2 is a versatile and reliable tool for the detection, activation, and manipulation of mouse T cells, recognized for its unique epitope specificity, high purity, and broad applicability in flow cytometry, immunohistochemistry, and functional assays. Its ability to partially cross-block other anti-CD3 antibodies underscores its value in multiplexed experimental designs and mechanistic studies of T cell biology. The dosing regimens of clone 500A2, an anti-mouse CD3ε antibody, vary significantly depending on the experimental mouse model and application. There is no universal standard, and dosing must be empirically optimized for each specific system and intended use. Key details about dosing regimens across mouse models:
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Conclusion: References & CitationsTechnical ProtocolsCertificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
