Anti-Mouse CD3ε – DyLight® 550

Anti-Mouse CD3ε – DyLight® 550

Product No.: C1730

[product_table name="All Top" skus="C1730"]

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Clone
500A2
Target
CD3ε
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG2a κ
Applications
FC

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Syrian Hamster
Immunogen
Purified Recombinant Mouse CD3ε (>98%)
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Yellow Laser (563 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 500A2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 500A2 recognizes an epitope on mouse CD3ε.
Antigen Distribution
CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Ligand/Receptor
Peptide antigen/MHC-complex.
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
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Immunology
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Signal Transduction

References & Citations

1. Havran, WM. et al. (1987) Nature 330:170
2. Allison, JW. et al. (1988) The T-Cell Receptor 33
Flow Cytometry
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.