Anti-Mouse CD3ε (Clone 500A2) – Purified

Home/Primary Monoclonal Antibodies/Surfacetag Mouse CD Markers/Anti-Mouse CD3ε (Clone 500A2) – Purified

Pricing & Details

Product No.C207
Clone
500A2
Protein
CD3ε
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Syrian Hamster
IgG2a κ
Applications
B
,
FC
,
IHC FF
Prod No.
Size
Price
Avail.
Qty
Add to cart
C207-50 µg
50 µg
$49.00
In stock
Max:
Min: 1
Step: 1
C207-500 µg
500 µg
$236.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Syrian Hamster
Immunogen
Mouse T-cell receptor
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 500A2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
IHC (Frozen) The suggested concentration for this 500A2 antibody in IHC staining on frozen tissue is 2.5 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.
B
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 500A2 recognizes an epitope on mouse CD3ε.
Antigen Distribution
CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein
Ligand/Receptor
Peptide antigen/MHC-complex.
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Immunology
.
Signal Transduction

References & Citations

Havran, W. M. et al. (1987) Nature 330:170
Allison, J. W. et al. (1988) The T-Cell Receptor 33
Flow Cytometry
IHC

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.