Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Syrian Hamster Immunogen Mouse T cell receptor Product Concentration 0.2 mg/ml Formulation This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze. Shipping Next Day 2-8°C Excitation Laser Blue Laser (493 nm) RRIDAB_2828792 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 500A2 antibody for staining cells in flow cytometry is ≤ 0.25 µg per million cells in 100 µl volume. Titration of the reagent is recommended for optimal performance for each application. Other Applications Reported In Literature ? IHC (Frozen) The suggested concentration for this 500A2 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone 500A2 recognizes an epitope on mouse CD3ε. Antigen Distribution CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes. Background CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition. Antigen DetailsProtein Ligand/Receptor Peptide antigen/MHC-complex. PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Immunology . Signal Transduction References & Citations1. Havran, WM. et al. (1987) Nature 330:170 2. Allison, JW. et al. (1988) The T-Cell Receptor 33 Technical Protocols |
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