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Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Goat Immunogen NS0-Derived Recombinant Mouse CD30 Ligand Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828541 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse CD30 Ligand this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CD30 Ligand is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Other Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse CD30 Ligand bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CD30 Ligand (at 500 ng/ml) is 3 - 10 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse CD30 Ligand recognizes Mouse CD30 Ligand. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background The CD30 ligand is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. Recombinant CD30L has been shown to co-stimulate T cells and to act as a mitogen for Hodgkin's disease derived cell lines. The receptor for CD30L, CD30/ TNFRSF8/CD153, is a type I cytokine receptor that is highly expressed by activated T cells, Hodgkin and Reed- Sternberg (H-RS) cells, and anaplastic large cell lymphoma cells. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis. Antigen DetailsProtein PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Croager, EJ. et al. (1997) Biochim. Biophys. Acta. 1353:231 2. Gruss, HJ. et al. (1994) Blood 83:1521 3. Wendtner, CM. et al. (1995) Cancer Res. 55:4157 4. Fischer, M. et al. (2006) J. Clin. Invest. 116:2748 5. Blazar, BR. et al. (2004) J. Immunol. 173:2933 Technical Protocols |
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