Anti-Mouse CD366 (Tim-3) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Mouse CD366 (Tim-3) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: T902

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Clone
RMT3-23
Target
Tim-3
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Havcr2
Isotype
IgG2a κ
Applications
IHC FF
,
PhenoCycler®

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Select Product Size
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CODEX® Details

Validation Notes
Clone RMT2-23 has been validated for use on CODEX® using mouse T-cell Sarcoma fresh frozen (FF) tissue.
Tissue Screened
T-cell Sarcoma
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Concentration
0.5 mg/ml
Immunogen
This antibody was produced using recombinant mouse TIM-3
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD366/Tim-3 (Clone RMT3-23) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2892983
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone RMT2-23 recognizes an epitope on mouse Tim-3.
Antigen Distribution
Tim-3 is expressed on activated Th1 and Tc1 lymphocytes, CD11b+ macrophages, Tregs, NK cells, and mast cells.
Background
Tim-3 antibody, clone RMT3-23, recognizes T cell immunoglobulin and mucin domain-containing protein 3 (TIM3, also known as CD366), a TIM family member of immunoregulatory proteins. Tim-3 is a 60kDa type I transmembrane protein with an extracellular immunoglobulin and mucin-like domain and a cytoplasmic tyrosine phosphorylation motif. Tim-3 is expressed by IFNg-producing Th1 CD4 and Tc1 (cytotoxic) CD8 T cells1, regulatory T cells (Tregs)2, myeloid cells3, NK cells4, and mast cells5. Tim-3 is an inhibitory molecule that limits Th1-mediated inflammatory diseases, including a model of central nervous system inflammation (experimental autoimmune encephalomyelitis, EAE)1, inflammatory bowel disease (IBD)6, and type I diabetes7. Tim-3 is also proposed to induce immunological tolerance7, promote Th1 apoptosis8, and regulate macrophage activation1. In addition, Tim-3 is upregulated on tumor-infiltrating lymphocytes, and coblockade of Tim-3 and immune checkpoint inhibitors, such as PD-1, is currently being investigated in clinical trials for the treatment of cancer9.

Antigen Details

Protein
Ligand/Receptor
Putative ligand on resting CD4+ lymphocytes
Function
May play a role in the development of immune responses and the development of Th1-mediated responses
PubMed
NCBI Gene Bank ID

References & Citations

1. Monney, L. et al. (2002)Nature 415, 536–541.
2. Gao, X. et al. (2012) PLOS ONE 7, e30676.
3. Anderson, A. C. et al. (2007) Science 318, 1141–1143.
4. Ndhlovu, L. C. et al. (2012) Blood 119, 3734–3743.
5. Phong, B. L. et al. J. (2015) Exp. Med. 212, 2289–2304.
6. Li, X. et al. (2010) Clin. Immunol. 134, 169–177.
7. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4, 1093–1101.
8. Zhu C, et al.. Nature Immunology. 2005;6:1245–1252.
IHC
PhenoCycler®
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.