Anti-Mouse CD4 (Clone YTS 191) – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.C3220
Clone
YTS 191
Protein
CD4
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Rat
IgG2b κ
Applications
Depletion
,
FC
,
IHC FF
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C3220-1.0 mg
1.0 mg
$95.00
In stock
Max:
Min: 1
Step: 1
C3220-5.0 mg
5.0 mg
$298.00
In stock
Max:
Min: 1
Step: 1
C3220-25 mg
25 mg
$800.00
In stock
Max:
Min: 1
Step: 1
C3220-50 mg
50 mg
$1,325.00
In stock
Max:
Min: 1
Step: 1
C3220-100 mg
100 mg
$1,998.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Unknown
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Other Applications Reported In Literature ?
Depletion
IHC (Frozen)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone YTS 191 recognizes an epitope on mouse CD4.
Antigen Distribution
CD4 is expressed on most thymocytes, MHC class II-restricted T cells, a subset of NKT cells, and subsets of dendritic cells and macrophages.
Background
CD4 antibody, clone YTS 191, recognizes CD4, a 58 kDa type I transmembrane glycoprotein of the Ig superfamily. CD4 is expressed by the majority of thymocytes, MHC class II-restricted T cells (helper T cells and immunosuppressive regulatory T cells), and subsets of natural killer T (NKT) cells, dendritic cells (DCs) and macrophages1-3. On T cells, CD4 is a co-receptor of the T cell receptor (TCR) and interacts with major histocompatibility complex (MHC) II molecules on antigen-presenting cells (APCs). CD4 contributes to T cell development and selection and enhances TCR-dependent signaling by up to 100-fold through the accumulation of Lck4-6. CD4 also contributes to the activation of NKT, macrophages, and DCs7,8.

Antigen Details

Protein
PubMed
Research Area
Immunology

References & Citations

1. Krijgsman D, et al. (2018) Front Immunol. 9:367
2. Esashi E, et al. (2003) J Immunol. 171(6):2773-7
3. Vremec D, et al. (2000) J Immunol. 164(6):2978-86
4. Li QJ, et al. (2004) Nat Immunol. 5:791–9
5. Janeway C. A. Jr. (1992) Annu Rev Immunol. 10:645–74
6. Germain RN. (2002) Nat Rev Immunol. 2(5):309-22
7. Thedrez A, et al. (2007) Blood. 110(1):251-8
8. Gibbings D & Befus AD. (2009) J Leukoc Biol. 86(2):251-9
Flow Cytometry
IHC
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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