Anti-Mouse CD54 (ICAM-1) (Clone YN1/1.7.4) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Mouse CD54 (ICAM-1) (Clone YN1/1.7.4) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C527

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Clone
YN1/1.7.4
Target
CD54
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Ly-47
Isotype
IgG2b κ
Applications
IHC FF
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone YN1/1.7.4 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.
Tissue Screened
Spleen
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD54 (Clone YN1/1.7.4) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2892850
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone YN1/1.7.4 recognizes an epitope on mouse CD54.
Antigen Distribution
CD54 is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes.
Background
ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.

Antigen Details

Protein
Ligand/Receptor
CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
PubMed
NCBI Gene Bank ID

References & Citations

1. Li, S. et al. (2009) Biochem. Biophys. Res. Commun. 381: 459
2. Wolf, S. et al. (2009) Pharmacol. Rep. 61: 22
3. Ozcan, U. et al. (2009) Arch Gynecol. Obstet.
IHC
PhenoCycler®
Products are for research use only. Not for use in diagnostic or therapeutic procedures.