Anti-Mouse CD90 (Thy-1) [T24/31] – Purified in vivo GOLD™ Functional Grade

Anti-Mouse CD90 (Thy-1) [T24/31] – Purified in vivo GOLD™ Functional Grade

Product No.: C392

[product_table name="All Top" skus="C392"]

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Clone
T24/31
Target
CD90
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
T25, CD90, Thy-1, Thy1.1, Thy1.2, Thy-1.2
Isotype
Rat IgG2b
Applications
Depletion
,
FA
,
FC
,
in vivo
,
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Recommended Isotype Controls
Recommended Dilution Buffer
Immunogen
Mouse Thy-1 protein
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this T24/31 antibody for staining cells in flow cytometry is ≤ 2.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Each investigator should determine their own optimal working dilution for specific applications.
Additional Applications Reported In Literature ?
WB Use at an assay dependent dilution.
Depletion
FA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
CloneT24/31 recognizes a non-polymorphic determinant on mouse CD90 (Thy1.1 and Thy1.2 alloantigens).
Background
CD90 is a 28-30 kD GPI-linked membrane glycoprotein and is part of the Ig superfamily. It interacts with CD45 in signal transduction. CD90 mediates adhesion of thymocytes to thymic stroma. It has been reported that CD90 binds with β2 and β3 integrins and is involved in the inhibition of hematopoietic stem cells proliferation and differentiation, as well as the regulation of cell adhesion and neurite outgrowth. It can be used as a marker for various stem cells, such as hematopoietic stem cells, and for the axonal processes of mature neurons. For use in FACS, CD90 is a popular surface marker for stem cells in combination with other markers such as CD34. There are two alleles for CD90 in mice that differ by one amino acid. The difference being that CD90.1 (Thy1.1) has an arginine and CD90.2 (Thy1.2) has a glutamine at position 108. CD90.2 is more prevalent and is expressed in most mice strains. CD90.1 is only expressed by a select few mice strains including AKR/J and PL strains. CD90.2 is a 25-35 kD GPI-anchored membrane glycoprotein. Like CD90, it is also in the Ig superfamily, interacts with CD45, and has involvement in signal transduction. The function of CD90.2 is thought to play roles in cognition, axon growth, T lymphocyte function, and apoptosis. CD90 acts as tumor suppressor for some tumors due to its action in upregulating thrombospondin, SPARC (osteonectin), and fibronectin. On the other hand, it has been suspected to aid in the spread of circulating melanoma cells. Regarding prostate cancer, CD90 has therapeutic potential for specific drug targeting due to its expression in cancer associated stroma, but not in normal stroma.
Antigen Distribution
CD90 is expressed by thymocytes, peripheral T cells, myoblasts, epidermal cells, and keratinocytes.
Ligand/Receptor
CD45
Function
The function of Thy1 has not been fully elucidated but is thought to play roles in regulation of cell adhesion, apoptosis, metastasis, inflammation, and fibrosis
PubMed
NCBI Gene Bank ID
Research Area
Immunology

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Clone T24/31 is a monoclonal antibody used in in vivo mouse studies primarily for the depletion of T lymphocytes, specifically targeting the CD90 (Thy-1) antigen on the surface of thymocytes and peripheral T cells.

Key details on its use:

  • Mechanism: T24/31 recognizes a non-polymorphic determinant on both Thy1.1 and Thy1.2 alloantigens, making it applicable to all mouse strains. This antigen is broadly expressed on thymocytes, peripheral T cells, and some intraepithelial T-lymphocytes.

  • Application in Depletion Studies: Researchers inject T24/31 in vivo (typically intraperitoneally) to induce T cell depletion. This technique enables experiments that probe the role of different T cell populations in immune responses, pathology, infection models (such as viral clearance), and immunotherapy research.

  • Experimental Protocol Example: One specific protocol describes injecting 250??g of anti-Thy1 (clone T24/31) intraperitoneally one day before infection to deplete T cells in BALB/c mice. This depletion was shown to increase the frequency of infected cells in tissues, demonstrating the role of T cells in viral control.

  • Antibody Properties: T24/31 is a rat IgG2b monoclonal antibody, typically formulated for low or ultra-low endotoxin content to minimize confounding inflammatory responses during in vivo use.

  • Dosage and Timing: Typical concentrations are 1.0–5.0?mg/ml, but the amount per mouse varies depending on the experimental requirements (e.g., 250??g per mouse for viral infection studies).

  • Additional Uses: Besides cell depletion, T24/31 may also be used in assays such as flow cytometry and functional tests, although its principal in vivo application is T lymphocyte depletion.

In summary, T24/31 is used to deplete T cells in mice for functional studies, disease modeling, and analysis of immune system components, with standard protocols involving intraperitoneal injections ahead of experimental interventions.

Commonly Used Antibodies or Proteins Alongside T24 (T24/31) in the Literature

There is limited direct evidence in the provided results regarding the specific scientific context or field in which “T24/31” antibodies are routinely paired with other antibodies or proteins, as the search results do not reference a widely recognized “T24/31” antibody pair (beyond T24 and a humanized version, RT24). Moreover, no literature directly associates T24 with a companion protein or antibody designated as “T31” in the context of co-immunoprecipitation, multiplex assays, or similar applications.

However, based on the most relevant papers found, here is what can be surmised about the T24 antibody and potential companion proteins in research:

T24 Antibody and Related Targets

  • T24 is a monoclonal antibody developed to recognize a cryptic epitope (residues 118–122) of human transthyretin (TTR), specifically detecting conformationally altered, aggregated, or fibrillar TTR, but not native, non-aggregated TTR.
  • RT24 is a humanized version of T24, exhibiting the same binding specificity and functional properties, including inhibition of TTR fibrillization and promotion of phagocytosis of TTR fibrils.
  • T24 and RT24 are discussed in the context of transthyretin amyloidosis (notably familial amyloid polyneuropathy, FAP), where TTR aggregation is pathogenic.

Companion Proteins and Antibodies in TTR Research

While T24/31 is not a standard pairing in the literature, research on TTR amyloidosis often involves:

  • Native (wild-type) TTR: Used as a negative control, since T24/RT24 do not bind non-aggregated TTR.
  • Mutant TTR variants (e.g., V30M, Y114C, A120S, V122I): Used to study the specificity and therapeutic potential of T24/RT24, as these mutants are associated with familial amyloidosis.
  • TTR fibrils and amyloid extracts from patients: Used to validate T24’s ability to recognize pathological aggregates.
  • General antibodies for TTR detection: While not specified in these papers, commonly used commercial anti-TTR antibodies (e.g., polyclonal anti-TTR, other monoclonals) are often employed in Western blotting, ELISA, and immunohistochemistry to detect total TTR, independent of aggregation state.

Broader Context: Western Blot and ELISA Companion Antibodies

  • Western blotting (WB) is a common technique to validate antibody specificity, often using loading control antibodies (e.g., anti-?-actin, anti-GAPDH) to confirm equal protein loading.
  • ELISA is used to quantify binding, where secondary antibodies conjugated to enzymes (e.g., HRP- or alkaline phosphatase-labeled anti-mouse or anti-human IgG) are standard for detection.

Table: Typical Companion Antibodies and Proteins in TTR/T24 Studies

Target/UseExample(s)Purpose/Nature
TTR (wild-type)Native TTRNegative control for T24/RT24 binding
TTR mutants (pathogenic)V30M, Y114C, V122I, etc.Model for disease, specificity testing
TTR fibrils/amyloidPatient-derived aggregatesValidate T24/RT24 binding to disease material
Loading controls?-actin, GAPDH antibodiesConfirm equal protein loading in WB
Secondary antibodiesHRP-anti-mouse, etc.Detect primary antibody in WB/ELISA

Summary

In the literature focused on T24 and transthyretin amyloidosis, T24 is primarily used alongside native and mutant TTR proteins, TTR fibrils/amyloids, and standard control antibodies (e.g., loading controls for Western blot, enzyme-conjugated secondaries for ELISA). There is no evidence in the provided results that “T31” is a recognized companion antibody or protein to T24 in this context. If “T24/31” refers to a specific experimental system or multiplex assay not described here, additional context would be needed to identify relevant companion antibodies or proteins.

In scientific literature, clone T24/31 most commonly refers to a monoclonal antibody targeting the Thy1 (CD90) antigen, widely used for T cell depletion in immunological research, particularly in mouse models. The key findings from citations involving T24/31 include:

  • Peripheral T Cell Depletion: T24/31 is frequently used to deplete peripheral T cells in vivo, allowing researchers to assess the role of tissue-resident T cells (TRM) versus circulating T cells during immune responses.
  • Experimental Insights on TRM Cells: Studies utilizing T24/31 have shown that, even after peripheral T cell depletion, residual CD4^+^ TRM cells in tissues (such as the lung) remain and are functionally sufficient to trigger localized immune responses—including airway hyperresponsiveness in asthma models.
  • Differentiation of Immune Responses: T24/31-based depletion helps distinguish secondary immune responses mediated by tissue-resident versus circulating T cells, elucidating their unique contributions to diseases like allergic asthma.
  • Specificity and Efficacy: T24/31 antibody treatment depletes the majority of circulating T cells, with >95% depletion of CD8^+^ T cells and about 75% of CD4^+^ T cells in the lung, while sparing tissue-resident fractions, confirming its effectiveness as a tool for studying compartment-specific immunity.

These findings have advanced understanding of how tissue-resident versus circulating T cells contribute to immune memory and pathology, particularly by leveraging the specificity of the T24/31 clone in experimental systems.

Dosing regimens for clone T24/31 in mouse models vary depending on the study design, mouse strain, therapeutic agent, administration frequency, and experimental endpoint. The search results do not explicitly reference a regimen for a clone specifically termed "T24/31"; instead, they reference the T24 cell line (a widely used human bladder cancer cell line) and its use in mouse xenograft models. If by "clone T24/31" you refer to a T24-derived subclone or experimental condition, clarification may be needed. Below is a synthesis of available data for T24 and related dosing regimens across studies using mouse models:

1. Chemotherapeutic or experimental drug dosing with T24 xenografts:

  • Typical practices involve inoculating mice (commonly nude mice) with T24 cells to establish tumors, followed by administration of anti-cancer agents at predetermined doses.
  • For example, one study used a fixed dose of 75 ?g per mouse for selected agents, citing a lack of established dose translation guidelines from cell culture to mice.
  • In multidrug combination screens, effective regimens involved drugs dosed in the range of nanograms to micrograms per mouse, with a focus on optimizing efficacy while minimizing toxicity.

2. Regimens in comparative pharmacology studies:

  • Antibody-drug conjugate (ADC) regimens in mice often mirror human clinical scaling (for context, not T24-specific): For instance, doses for anti-Nectin-4 ADC enfortumab vedotin ranged from 6 to 15 mg/kg in mice, approximating scaled human dosing, and administration frequency was adjusted depending on the agent and desired pharmacodynamic endpoints.

3. Dosing intervals and sample timing:

  • Studies may vary in the timing and frequency of drug administration; common regimens include single dosing or repeated administration every few days, depending on drug PK/PD (pharmacokinetic/pharmacodynamic) properties and therapeutic objectives.

4. Mouse strain, tumor implantation technique, and outcome measures:

  • Variations in mouse strains (such as nude or immunodeficient lines), the number of cells injected, and route of administration (e.g., subcutaneous or orthotopic) can influence dosing regimens. These parameters are standardized within each study protocol but can differ across the literature.

Summary Table: Reported dosing examples in T24 mouse studies

Study ApplicationMouse StrainT24 SourceDose ExampleFrequency/Duration
Multidrug optimizationNudeT24 cell line xenograft75 ?g/mouseAs per protocol (varied)
Compound efficacyNot specifiedT24 cell line inoculation0.125–0.5 ?M (in vitro), in vivo not specifiedNot stated (varied)
ADC/Nectin-4 studiesImmunodeficientHT1376 (urothelial, not T24)6, 9, or 15 mg/kgPer dose, intervals specified

Current literature demonstrates no universal dosing regimen for T24/31 across mouse models; protocols are tailored to the cell line, agent, and hypothesis under investigation. Doses range considerably, and specific regimens depend on intended therapeutic effect, toxicity, and outcome measures.

If "clone T24/31" refers to a specific subclone or engineered cell line, or if a precise dosing protocol is needed, please specify the context (e.g., target drug, therapeutic class, or model system) for a more detailed answer.

References & Citations

Dennert, G. et al. (1980) Cell Immunol. 53:350
Depletion
FA
Flow Cytometry
in vivo Protocol
General Western Blot Protocol

Certificate of Analysis

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.