Anti-Mouse CTLA-4 (CD152) – Biotin

Anti-Mouse CTLA-4 (CD152) – Biotin

Product No.: C400

[product_table name="All Top" skus="C400"]

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Target
CTLA-4
Product Type
Polyclonal Antibody
Alternate Names
CD152, Cytotoxic T Lymphocyte-Associated Antigen-4, Ly-56, CELIAC3, GSE, IDDM12, CELIAC3, GSE, IDDM12
Applications
ELISA Det
,
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
NS0-Derived Recombinant Mouse CTLA-4 Extracellular Domain
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse CTLA-4 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CTLA-4 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Detection: Suitable for use at concentration of 0.1-0.4 µg/mL. Use in combination with: Capture Reagent: Mouse CTLA‑4 Antibody. Standard: Recombinant Mouse CTLA-4 Fc Chimera Protein.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse CTLA-4 recognizes Mouse CTLA-4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
CTLA-4 is a 33 kD member of the Ig superfamily similar to CD28 in amino acid sequence, structure, and genomic organization. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. It is involved in the development of protective immunity and thymocyte regulation, in addition to the induction and maintenance of immunological tolerance. CTLA-4 has therapeutic potential both as an agonist to reduce immune activity, and an antagonist to increase immune activity.
Antigen Distribution
CTLA-4 is expressed on activated T and B lymphocytes.

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Layrisse, Z. et al. (2009) Hum Immunol. 70(7):532-5.
2. Mak, TW. et al. (1995) Science 270: 985
3. Delneste, Y. et al. (1999) Eur. J. Immunol 29: 3596
4. Vijayakrishnan, L. et al. (2004) Immunity 20: 563
ELISA Det
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.