Anti-Mouse DPPIV/CD26

Anti-Mouse DPPIV/CD26

Product No.: D357

[product_table name="All Top" skus="D357"]

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Clone
155202
Target
Dipeptidyl Peptidase IV
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD26, DPP IV
Isotype
IgG2a
Applications
CyTOF®
,
ELISA Indirect
,
FC

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
NS0-Derived Recombinant Mouse DPPIV Ectodomain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:R380. The detection sensitivity of this indirect ELISA for Mouse DPPIV is approximately 5 ng/well.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing DPPIV. A suggested method would be to stain cells expressing DPPIV with 0.25 µg per 1 - 2.5 x 105 cells in a total staining volume of ≤200 µl followed by PN:R485.
Additional Applications Reported In Literature ?
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Specificity
Rat Anti-Mouse Dipeptidyl Peptidase IV (DPPIV) (Clone 155202) recognizes an epitope on Mouse DPPIV. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Details

NCBI Gene Bank ID
CyTOF®
Indirect Elisa Protocol
Flow Cytometry

Certificate of Analysis

Formats Available

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Prod No.
Description
D357
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.