Anti-Mouse EphA8 – Biotin

Anti-Mouse EphA8 – Biotin

Product No.: E284

[product_table name="All Top" skus="E284"]

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Target
EphA8
Product Type
Polyclonal Antibody
Alternate Names
EPH Receptor A8, EEK, HEK3, KIAA1459
Applications
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse EphA8 (Accession # O09127)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse EphA8 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse EphA8 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E205.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse EphA8 recognizes Mouse EphA8. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
EPH receptor A8, also known as EPHA8 is a receptor tyrosine kinase of the Eph family1 that functions exclusively as an glycosyl phosphatidylinositol (GPI)-linked ephrin ligand-dependent receptor protein tyrosine kinase.2 EPHA8 plays a role in axonal path finding during development of the mammalian nervous system.3

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Muir, EM. et al. (1999) Brain Res Mol Brain Res. 74: 225
2. Choi, S. et al. (1999) Molecules and Cells 9: 440
3. Barbacid, M. et al. (1997) EMBO J 16: 3106
General Western Blot Protocol
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Prod No.
Description
E238
E284
Products are for research use only. Not for use in diagnostic or therapeutic procedures.