Anti-Mouse Ephrin-B1 – Biotin
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen NSO-Derived Recombinant Mouse Ephrin-B1 Extracellular Domain (Accession # AAA53231) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830059 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse Ephrin-B1 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Ephrin-B1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse Ephrin-B1 recognizes Mouse Ephrin-B1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Ephrin-B1 (EFNB1) is a glycoprotein that is expressed on neuroepithelial cells1 and post-synaptically on dendritic spines in the cortex, supporting a role in synaptic plasticity in this region.2 Ephrin-B1 is also highly expressed on developing peripheral nerve fibers including auditory and vestibular (statoacoustic) and dorsal root ganglion nerve fibers and provides guidance signals to these cells.3 Ephrin-B1 participates in cell-cell repulsive and attractive signaling together with its Eph receptor.4 Tyrosine phosphorylation of ephrin-B1 promotes invasion of cancer cells in vivo and is a potential therapeutic target in some types of gastrointestinal cancers.5 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Stuckmann, I. et al. (2001) J Neurosci. 21: 2726 2. Rodger, J. et al. (2009) Brian Res. 1247: 50 3. Gray, NA. et al. (2002) European J Neurosci. 16: 1499 4. Iwatsubo, T. et al. (2006) Mol Neurodegeneration 1: 2 5. Sakai, R. et al. (2007) Am J Pathol. 171: 68 Technical Protocols |
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